Characterization of 10 novel microsatellite loci and cross-amplification of two loci in the snapping turtle (Chelydra serpentina)
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We use 454 (“shotgun”) sequencing to obtain a partial genomic library for the snapping turtle (Chelydra serpentina). We characterize ten microsatellite loci from these sequences and test cross-amplification of loci originally developed for the alligator snapping turtle (Macrochelys temminckii). We genotype 127 individuals from Ontario at twelve loci. The number of alleles per locus ranges from 1 to 14; heterozygosity ranges from 0.157 to 0.850. These loci will be used to study population genetic structure in this long-lived reptile and may cross-amplify in two closely related species.
KeywordsMicrosatellite Chelydra serpentina Macrochelys temminckii
This project was supported by a Canada Collection grant from Wildlife Preservation Canada (WPC) to C.D. and a National Science and Engineering Research Council (NSERC) Discovery Grant (A3148) to R.W.M. Genotyping costs were offset by the generous assistance of the Schad Foundation. C.D. and I.D. are funded by Canada Graduate Scholarships from NSERC; A.L. is funded by WPC through a Science Horizons grant from Environment Canada. We thank Dr. C. Kyle, E. Kerr and M. Harnden at the NRDPFC, Trent University, for assistance with 454 sequencing. Sample collection was conducted with the permission of the Government of Ontario and Ontario Parks and followed Animal Use Protocol 2010-14 (Royal Ontario Museum).
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