Fifty-nine microsatellite markers for hybrid classification studies involving endemic Florida Mottled Duck (Anas fulvigula fulvigula) and invasive Mallards (A. platyrhynchos)
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Endemic Florida Mottled Duck (Anas fulvigula fulvigula) appear to be hybridizing introgressively with domesticated Mallards (A. platyrhynchos), which are frequently released or escape captivity and have established feral populations throughout Florida. To investigate this possible conservation threat, we isolated and characterized 59 polymorphic loci from an enriched Florida Mottled Duck microsatellite library and performed cross-amplification assays with Mallard specimens. Average numbers of alleles per locus were 6.0 (ranging 2–23) and 5.6 (ranging 2–15) for A. fulvigula and A. platyrhynchos, respectively; estimates of observed/expected heterozygosity were 0.54/0.63 and 0.52/0.64. Markers developed in this study will be used in conjunction with existing markers to robustly classify hybrids and to assess and monitor the genetic dynamics of introgression between these waterfowl species.
KeywordsFlorida Mottled Duck Anas fulvigula Mallard Anas platyrhynchos Microsatellite markers Hybridization
This study was supported and funded by the Florida Fish and Wildlife Conservation Commission; substantial funding was also provided by the United States Fish and Wildlife Service (Region 4). We gratefully acknowledge A. Engilis and I. Engilis for their assistance and expertise. This study would not have been possible without the efforts of J. Millikin, K. Wiley, D. Blood, J. McGrady, S. Rockwood, J. Albury, E. Harmon, M. McMunigal, and B. Walker, who collected many of the study specimens. We also thank the many hunters at T.M Goodwin WMA and Stormwater Treatment Areas 1 W and 5, who donated tissues and specimens, and staff members at Walt Disney World, who assisted with captures on their property.
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