Conservation Genetics Resources

, Volume 4, Issue 3, pp 677–679

Isolation and development of 15 new, polymorphic microsatellite loci for an unusual, endemic African earwig (Hemimerus vosseleri)

  • K. C. Murdoch
  • J. D. Keyyu
  • E. D. Karimuribo
  • K. A. Feldheim
  • N. J. Cordeiro
Technical Note
  • 117 Downloads

Abstract

Eighteen microsatellite loci were isolated from the Hemimerus vosseleri earwig, an insect species endemic to the Eastern Arc Mountains, and that lives on African Giant Pouched Rat (Cricetomysgambianus). Alleles ranged between two and 12 per locus, with expected heterozygosity ranging from 0.185 to 0.883 and observed heterozygosity ranging from 0.200 to 0.750. Fifteen of 18 loci were polymorphic. These microsatellite markers will be used to study the population structure of Hemimerusvosseleri among different rat hosts, which will likely enhance the conservation of this unique and unusual rodent-insect partnership.

Keywords

Earwig Hemimerus vosseleri Microsatellite markers Mutualism Tanzania 

References

  1. Ashford RW (1970) Observations on the biology of Hemimerus talpoides (Insecta: Dermaptera). J Zool Soc Lond 162:413–418CrossRefGoogle Scholar
  2. Brooks TM, Mittermeier RA, Mittermeier CG, da Fonseca GAB, Rylands AB, Konstant WR, Flick P, Pilgrim P, Oldfield S, Magin G, Hilton-Taylor C (2002) Habitat loss and extinction in the hotspots of biodiversity. Conserv Biol 16:909–923CrossRefGoogle Scholar
  3. Dunn RR, Harris NC, Colwell RK, Koh LP, Sodhi NS (2009) The sixth mass coextinction: are most endangered species parasites and mutualists? Proc R Soc Lond [Biol] 276:3037–3045CrossRefGoogle Scholar
  4. Glenn TC, Schable NA (2005) Isolating microsatellite DNA loci. Methods Enzymol 395:202–222PubMedCrossRefGoogle Scholar
  5. Marshall TC, Slate J, Kruuk LEB, Pemberton JM (1998) Statistical confidence for likelihood-based paternity inference in natural populations. Mol Ecol 7:639–655PubMedCrossRefGoogle Scholar
  6. Myers N, Mittermeier RA, Mittermeier CG, da Fonseca GAB, Kent J (2000) Biodiversity hotspots for conservation priorities. Nature 403:853–858PubMedCrossRefGoogle Scholar
  7. Nakata S, Maa TC (1974) A review of the parasitic earwigs. Pacific Insects 16:307–374Google Scholar
  8. Raymond ML, Rousset F (1995) An exact test for population differentiation. Evolution 49:1280–1283CrossRefGoogle Scholar
  9. Rehn JAG, Rehn JWH (1935) A study of the genus Hemimerus (Dermaptera, Hemimerina, Hemimeridae). Proc Acad Natural Sci Phil 87:457–508Google Scholar
  10. Rice WR (1989) Analyzing tables of statistical tests. Evolution 43:223–225CrossRefGoogle Scholar
  11. Schuelke M (2000) An economic method for the fluorescent labeling of PCR fragments. Nature Biotechnol 18:233–234CrossRefGoogle Scholar
  12. Waage JK (1979) The evolution of insect and vertebrate association. Biol J Linnean Soc 12:188–216CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • K. C. Murdoch
    • 1
    • 2
  • J. D. Keyyu
    • 3
  • E. D. Karimuribo
    • 4
  • K. A. Feldheim
    • 2
  • N. J. Cordeiro
    • 1
    • 2
  1. 1.Department of Biological, Chemical, and Physical SciencesRoosevelt UniversityChicagoUSA
  2. 2.The Field MuseumChicagoUSA
  3. 3.Tanzania Wildlife Research InstituteArushaTanzania
  4. 4.Department of Veterinary Medicine and Public HealthSokoine University of AgricultureMorogoroTanzania

Personalised recommendations