Conservation Genetics Resources

, Volume 4, Issue 3, pp 621–624

Development and characterization of 13 new, and cross amplification of 3, polymorphic nuclear microsatellite loci in the common myna (Acridotheres tristis)

  • Cécile Berthouly-Salazar
  • Phillip Cassey
  • Bettine Jansen van Vuuren
  • Berndt Janse van Rensburg
  • Cang Hui
  • Michael G. Gardner
  • Johannes J. Le Roux
Technical Note

Abstract

We used a next generation sequencing (NGS) approach to screen for genome-wide nuclear microsatellite loci in the common (Indian) myna, Acridotheres tristis. In addition, markers previously developed for other Sturnidae species were tested for cross-amplification in A. tristis. In total, we identified 20 loci from NGS data and tested 26 loci for cross-amplification. Out of all loci (NGS developed and cross-amplified), 16 unlinked loci showed polymorphism, ranging from 2 to 9 alleles per locus. Test individuals were obtained from the invasive ranges of common myna in South Africa. Overall, expected and observed heterozygosities ranged from 0.089 to 0.802 and from 0.094 to 0.906, respectively. These markers will be used to shed light on invasion genetics and landscape-scale dynamics of invasive A. tristis in South Africa.

Keywords

Acridotheres tristis Common myna Invasive species Microsatellite 

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Cécile Berthouly-Salazar
    • 1
  • Phillip Cassey
    • 2
  • Bettine Jansen van Vuuren
    • 3
  • Berndt Janse van Rensburg
    • 4
  • Cang Hui
    • 1
  • Michael G. Gardner
    • 2
    • 5
  • Johannes J. Le Roux
    • 1
  1. 1.DST-NRF Centre of Excellence for Invasion Biology, Department of Botany and ZoologyStellenbosch UniversityMatielandSouth Africa
  2. 2.Australian Centre for Evolutionary Biology and Biodiversity, School of Earth and Environmental ScienceUniversity of AdelaideAdelaideAustralia
  3. 3.DST-NRF Centre of Excellence for Invasion Biology, Department of ZoologyUniversity of JohannesburgAuckland ParkSouth Africa
  4. 4.DST-NRF Centre of Excellence for Invasion Biology, Department of Zoology and EntomologyUniversity of PretoriaHatfieldSouth Africa
  5. 5.School of Biological SciencesFlinders UniversityAdelaideAustralia

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