Conservation Genetics Resources

, Volume 4, Issue 3, pp 603–604 | Cite as

TaqMan assays for species identification of the red squirrel (Sciurus vulgaris) and the grey squirrel (Sciurus carolinensis)

  • Denise B. O’Meara
  • Peter D. Turner
  • Lee Coffey
  • Catherine O’Reilly
Technical Note

Abstract

We have developed TaqMan based assays for species-specific identification of two species of squirrel found in the British Isles, the native red squirrel (Sciurus vulgaris) and the introduced north American grey squirrel (Sciurus carolinensis). These assays correctly identified tissue and hair samples of both species and there was no cross-species amplification. This is a useful method for non-invasive surveys to help conserve the red squirrel and manage the spread of the grey squirrel in the UK and Ireland.

Keywords

Real-time PCR Non-invasive Hair-tubes Mitochondrial DNA 

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Denise B. O’Meara
    • 1
  • Peter D. Turner
    • 1
  • Lee Coffey
    • 1
  • Catherine O’Reilly
    • 1
  1. 1.Department of Chemical and Life SciencesWaterford Institute of TechnologyWaterfordIreland

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