Conservation Genetics Resources

, Volume 4, Issue 3, pp 545–548 | Cite as

Microsatellite marker isolation and development for the giant Pacific Octopus (Enteroctopus dofleini)

  • R. K. Toussaint
  • G. K. Sage
  • S. L. Talbot
  • D. Scheel
Technical Note


We isolated and developed 18 novel microsatellite markers for the giant Pacific octopus (Enteroctopus dofleini) and examined them for 31 individuals from Prince William Sound (PWS), Alaska. These loci displayed moderate levels of allelic diversity (averaging 11 alleles per locus) and heterozygosity (averaging 65%). Seven loci deviated from Hardy–Weinberg Equilibrium (HWE) due to heterozygote deficiency for the PWS population, although deviations were not observed for all these loci in other populations, suggesting the PWS population is not in mutation-drift equilibrium. These novel microsatellite loci yielded sufficient genetic diversity for potential use in population genetics, individual identification, and parentage studies.


Microsatellite markers Enteroctopus dofleini Population genetics 



We thank Sarah Sonsthagen and C. Roman Dial for laboratory and analytical assistance, and Gordon Scott, Reid Brewer, Robert Berceli, and Elisa Russ for samples. Henry Tomingas provided sampling equipment and boat use. Funding was provided by the At-Sea Processors Association Pollock Conservation Cooperative through Alaska Pacific University, the Alaska Space Grant Program, USGS Alaska Science Center, and Alaska Pacific University. Mention of trade names or commercial products does not constitute endorsement or recommendation for use.


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Copyright information

© Springer Science+Business Media B.V. (outside the USA) 2011

Authors and Affiliations

  • R. K. Toussaint
    • 1
  • G. K. Sage
    • 2
  • S. L. Talbot
    • 2
  • D. Scheel
    • 1
  1. 1.Alaska Pacific UniversityAnchorageUSA
  2. 2.United States Geological SurveyAnchorageUSA

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