Development of ten microsatellite markers for Arisaema minus (Araceae), a vulnerable Japanese herb species
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Arisaema minus is a vulnerable perennial herb species endemic to Japan. We isolated and characterized ten microsatellite loci in this species. The number of alleles per locus ranged from 3 to 28, with an average of 12.8. The observed heterozygosities ranged from 0.472 to 0.861 with an average of 0.703, and the expected heterozygosities ranged from 0.486 to 0.962 with an average of 0.779. The total exclusionary powers of all ten loci, when no parental information existed or when one parent was known, were 0.99940154 and 0.99998730, respectively, indicating the high resolution of these microsatellite loci. This marker set is potentially useful for conservation genetics, reproductive studies, and the development of effective A. minus conservation programs.
KeywordsArisaema minus Conservation genetics Local endemic Microsatellite
We thank Ryohei G. Ohara, Kumiko Michimoto, Ryo Suzuki, and Yuko Nagata for their assistance with field sampling. This work was supported by the Global Environment Research Fund (D-0903) of the Ministry of the Environment, Japan.
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