Conservation Genetics Resources

, Volume 4, Issue 2, pp 495–497 | Cite as

Development of ten microsatellite markers for Arisaema minus (Araceae), a vulnerable Japanese herb species

  • Chihiro Fukada
  • Shingo Kaneko
  • Masashi Yokogawa
  • Tomiki Kobayashi
  • Atushi Ushimaru
  • Yuji Isagi
Technical Note
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Abstract

Arisaema minus is a vulnerable perennial herb species endemic to Japan. We isolated and characterized ten microsatellite loci in this species. The number of alleles per locus ranged from 3 to 28, with an average of 12.8. The observed heterozygosities ranged from 0.472 to 0.861 with an average of 0.703, and the expected heterozygosities ranged from 0.486 to 0.962 with an average of 0.779. The total exclusionary powers of all ten loci, when no parental information existed or when one parent was known, were 0.99940154 and 0.99998730, respectively, indicating the high resolution of these microsatellite loci. This marker set is potentially useful for conservation genetics, reproductive studies, and the development of effective A. minus conservation programs.

Keywords

Arisaema minus Conservation genetics Local endemic Microsatellite 

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Chihiro Fukada
    • 1
  • Shingo Kaneko
    • 2
  • Masashi Yokogawa
    • 2
  • Tomiki Kobayashi
    • 3
  • Atushi Ushimaru
    • 1
  • Yuji Isagi
    • 2
  1. 1.Graduate School of Human Development and EnviromentKobe UniversityHyogoJapan
  2. 2.Laboratory of Forest Biology, Division of Forest and Biomaterials Science, Graduate School of AgricultureKyoto UniversityKyotoJapan
  3. 3.The Society for Flora of HyogoAkashiJapan

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