Advertisement

Conservation Genetics Resources

, Volume 4, Issue 2, pp 423–428 | Cite as

Isolation and characterization of 55 novel microsatellite markers for the pink-footed goose (Anser brachyrhynchus)

  • Kristina Noreikiene
  • Amber G. F. Teacher
  • Jesper Madsen
  • Phillip Gienapp
Technical Note

Abstract

Here we present 55 new microsatellite markers isolated from the pink-footed goose (Anser brachyrhynchus), using next generation sequencing. Of these, 44 markers were found to be polymorphic, and the number of alleles ranged from two to ten with a mean of 4.23 alleles per locus. Mean observed heterozygosity was 0.44, and 33 polymorphic markers were in Hardy–Weinberg equilibrium in our sample population. These newly developed molecular markers could prove to be a useful tool for further studies of pink-footed goose populations.

Keywords

Anser Pink-footed goose Co-dominant markers Microsatellite Next generation sequencing 

Notes

Acknowledgments

We thank Minna Ruokonen (University of Oulu) for extracting the DNA and Marika Karjalainen for help in the laboratory. Permits for catching the birds and DNA sampling were issued from the Governor of Svalbard and the Copenhagen Bird Ringing Centre to JM. This study was founded by a grant from the Academy of Finland to PG (grant no. 1123565).

References

  1. IPCC (2007) Climate change 2007: synthesis report. In: Core Writing Team, Pachauri RK, Reisinger A (eds) Contribution of working groups I, II and III to the 4th assessment report of the intergovernmental panel on climate change. IPCC, GenevaGoogle Scholar
  2. Knudsen E, Lindén A, Both C, Jonzén N, Pulido F, Saino N, Sutherland WJ, Bach LA et al (2011) Challenging claims in the study of migratory birds and climate change. Biol Rev (in press)Google Scholar
  3. Madsen J, Cracknell, G, Fox AD (eds) (1999) Goose populations of the Western Palearctic. A review of status and distribution. Wetlands International Publication No. 48. Wetlands International, Wageningen, The Netherlands. National Environmental Research Institute, Rønde, 344 ppGoogle Scholar
  4. Meglécz E, Costedoat C, Dubut V, Gilles A, Malausa T, Pech N, Martin JF (2010) QDD: a user-friendly program to select microsatellite markers and design primers from large sequencing projects. Bioinformatics 26:403–404PubMedCrossRefGoogle Scholar
  5. Park SDE (2001) Trypanotolerance in West African cattle and the population genetic effects of selection. University of Dublin, DublinGoogle Scholar
  6. Rousset F (2008) Genepop’007: a complete reimplementation of the Genepop software for Windows and Linux. Mol Ecol Res 8:103–106CrossRefGoogle Scholar
  7. Ruokonen M, Aarvak T, Madsen J (2005) Colonization history of the high-arctic pink-footed goose Anser brachyrhynchus. Mol Ecol 14:171–178PubMedCrossRefGoogle Scholar
  8. Taggart JB, Hynes RA, Prodöuhl PA, Ferguson A (1992) A simplified protocol for routine total DNA isolation from salmonid fishes. J Fish Biol 40:963–965CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Kristina Noreikiene
    • 1
  • Amber G. F. Teacher
    • 1
  • Jesper Madsen
    • 2
  • Phillip Gienapp
    • 1
    • 3
  1. 1.Department of BiosciencesUniversity of HelsinkiHelsinkiFinland
  2. 2.Department of BioscienceUniversity of AarhusRoskildeDenmark
  3. 3.Institute for Evolution and EcologyUniversity of TübingenTübingenGermany

Personalised recommendations