Conservation Genetics Resources

, Volume 4, Issue 2, pp 391–393 | Cite as

An efficient molecular sexing of the vulnerable Chinese egret (Egretta eulophotes) from faeces samples

Technical Note


A species-specific primer set has been developed and used with a preamplification and multiplex PCR method for molecular sexing from the faeces of the vulnerable Chinese egret (Egretta eulophotes). This new assay produced one common fragment in both females and males (approximately 230 bp) and one specific fragment in females (about 136 bp). A highly efficient sex identification result (97.7%, 171/175 faeces samples) and great versatility on seven other ardeid species suggests that this improved molecular approach is a sensitive sexing tool for the Chinese egret and other ardeid species. It will facilitate further studies on their population sex structure using non-invasive genetic samples.


Sex identification Faecal DNA Egretta eulophotes Vulnerable species Ardeidae 



We thank Zeng Wang, Zhijun Huang and Hang Gao for their helps in sampling and thank Yufei Dai and Zhenni Peng for their assists in lab work. This work was supported by the National Natural Science Foundation of China (Grant Nos. 30970380,31000963 and J1030626) and by the Fujian or Henan Natural Science Foundation of China (2010Y2007,2010J05084, 2011J01247 and 102102110180).


  1. Bozarth CA, Alva-Campbell YR, Ralls K, Henry TR, Smith DA, Westphal MF, Maldonado JE (2010) An efficient noninvasive method for discriminating among faeces of sympatric North American canids. Conserv Genet Resour 2:173–175CrossRefGoogle Scholar
  2. Fang WZ, Lin QX, Chen XL, Lin JH (2011) Nestling diet of the vulnerable Chinese egret on offshore islands in southern China. Waterbirds 34:246–251CrossRefGoogle Scholar
  3. Griffiths R, Double MC, Orr K, Dawson RJG (1998) A DNA test to sex most birds. Mol Ecol 7:1071–1075PubMedCrossRefGoogle Scholar
  4. Huang XH, Zhou XP, Chen M, Fang WZ, Chen XL (2010) Isolation and characterization of microsatellite loci in vulnerable Chinese egret (Egretta eulophotes: Aves). Conserv Genet 11:1211–1214CrossRefGoogle Scholar
  5. Huang XH, Zhou XP, Lin QX, Peng ZN, Fang WZ, Chen XL (2011) A novel multiplex PCR assay for species identification in the Chinese egret (Egretta eulophotes) and little egret (E. garzetta). Conserv Genet Resour. doi: 10.1007/s12686-011-9466-8
  6. IUCN (2010) IUCN red list of threatened species. Accessed 28 May 2011
  7. Jiang S, Chen XL (2006) Sex identification of ardeid birds by amplifying sex gene sequence. Chin J Xiamen Univ (Natural Science) 45:152–155Google Scholar
  8. Lachish S, Passmore A, Jones M (2011) A new PCR assay for reliable molecular sexing of endangered Tasmanian devils (Sarcophilus harrisii) from non-invasive genetic samples. Conserv Genet Resour 3:279–281CrossRefGoogle Scholar
  9. Pérez T, Vāzquez JF, Quirós F, Dominguez A (2011) Improving non-invasive genotyping in capercaillie (Tetrao urogallus): redesigning sexing and microsatellite primers to increase efficiency on faeces samples. Conserv Genet Resour 3:483–487CrossRefGoogle Scholar
  10. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882PubMedCrossRefGoogle Scholar
  11. Wang Z, Zhou XP, Lin QX, Fang WZ, Chen XL (2011) New primers for sex identification in the Chinese egret and other ardeid species. Mol Ecol Resour 11:176–179PubMedCrossRefGoogle Scholar
  12. Wei GA, Yin ZH, Lei FM (2005) Copulations and mate guarding of the Chinese egret. Waterbirds 28:527–530CrossRefGoogle Scholar
  13. Yamashiro A, Yamashiro T, Baba M, Endo A, Kamada M (2010) Species identification based on the faecal DNA samples of the Japanese serow (Capricornis crispus). Conserv Genet Resour 2:409–414CrossRefGoogle Scholar
  14. Zhou XP, Wang YQ, Chen XL, Lin QX, Fang WZ, Wei D (2008) A set of primer pairs for amplifying the complete mitochondrial DNA of endangered Chinese egret (Aves, Ardeidae, Egretta eulophotes). Mol Ecol Resour 8:412–414PubMedCrossRefGoogle Scholar
  15. Zhou XP, Fang WZ, Chen XL (2010) Mitochondrial DNA diversity of the vulnerable Chinese egret (Egretta eulophotes) from China. J Ornithol 151:409–414CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  1. 1.Key Laboratory of Ministry of Education for Coast and Wetland Ecosystems, School of Life SciencesXiamen UniversityXiamenPeople’s Republic of China
  2. 2.College of Life SciencesJiaying UniversityGuangdongPeople’s Republic of China

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