Isolation and characterization of twelve polymorphic microsatellite loci for investigating an extreme case of microgeographical variation in an island bird (Zosterops borbonicus)
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Twelve polymorphic microsatellite loci were characterized in order to investigate an extreme case of microgeographical variation in an island-dwelling passerine (Zosterops borbonicus). Variation was examined in two distinct natural populations of 27 individuals each. The loci displayed 5–20 alleles, with observed heterozygosities ranging between 0.33 and 0.93. All but one loci (Z16) conformed to Hardy–Weinberg equilibrium. None of the pairwise comparisons among loci showed significant linkage disequilibrium after sequential Bonferonni correction. Successful cross-species amplification in additional Zosterops species and other passerines suggests their broad applicability.
KeywordsBird Island Microsatellites White-eye Zosterops
Funding was provided by the IFB, the ANR Biodiversity Program, and the SYNTHESYS Program (http://www.synthesys.info/) of the European Community Research Infrastructure Action under the FP7 “Capacities” Programme at the National Museum of Natural Sciences (CSIC) in Madrid, Spain. We are grateful to Benoit Lequette, Marc Salamolard and Dominique Strasberg for facilitating sample collection, and to Ben Warren for providing blood samples.
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