Abstract
In the present study, a microsatellite-enriched genomic library was constructed and primers for 14 microsatellite loci were designed for Xylocopa frontalis. Twenty unrelated individuals were screened. All loci were polymorphic and the number of alleles per locus ranged from 6 to 17 (x = 10.43). Observed (Ho) and expected (He) heterozygosities ranged from 0.350 to 0.950 and 0.674 to 0.898, respectively. All loci were in Hardy–Weinberg equilibrium, except one. The microsatellite loci described in this study will contribute towards general biology studies of X. frontalis, intranidal genetic relationships and nest management for the pollination of passion fruit.
References
Augusto SC, Garófalo CA (2004) Nesting biology and social structure of Euglossa (Euglossa) townsendi Cockerell (Hymenoptera, Apidae, Euglossini). Insect Soc 51:400–409
Augusto SC, Garófalo CA (2009) Bionomics and sociological aspects of Euglossa fimbriata (Apidae, Euglossini). Genet Mol Res 8:525–538
Augusto SC, Garófalo CA (2010) Task allocation and interactions among females in Euglossa carolina nests (Hymenoptera, Apidae, Euglossini). Apidologie 42:162–173. doi:10.1051/apido/2010040
Billotte N, Lagoda PJR, Risterucci AM, Baurens FC (1999) Microsatellite-enriched libraries: applied methodology for the development of SSR markers in tropical crops. Fruits 54:277–288
Brito RM, Francisco FO, Domingues-Yamada AMT, Gonçalves PHP, Pioker FC, Soares AEE, Arias MC (2009) Characterization of microsatellite loci of Tetragonisca angustula (Hymenoptera, Apidae, Meliponini). Conserv Genet Resour 1:183–187
Camillo E (2003) Polinização de maracujá. Holos Editora, Ribeirão Preto
Camillo E, Garófalo CA (1982) On the bionomics of Xylocopa frontalis (Oliver) and Xylocopa grisescens (Lepeletier) in southern Brazil. I— nest construction and biological cycle. Rev Brasil Biol 42:571–582
Camillo E, Garófalo CA (1989) Social organization in reactivated nests of three species of Xylocopa (Hymenoptera, Anthophoridae) in southeastern Brasil. Insect Soc 36:92–105
Camillo E, Garófalo CA, Muccillo G (1986) On the bionomics of Xylocopa suspecta (Moure) in southern Brazil: nest construction and biological cycle (Hymenoptera: Anthophoridae). Rev Brasil Biol 46:383–393
Carvalho SM (2011) Diversidade de abelhas e vespas solitárias (Hymenoptera, Apoidea) que nidificam em ninhos-armadilha disponibilizados em áreas de Cerrado e em fragmentos próximos de Mata Estacional Semidecidual—MG. Dissertation, Universidade Federal de Uberlândia
Chaves-Alves TM (2009) Nidificação de Xylocopa spp. (Apidae, Xylocopini) em ninhos-armadilha em áreas de Cerrado do Triângulo Mineiro. Dissertation, Universidade Federal de Uberlândia
Francisco FO (2009) FOFpop v.2.0, a pool of Microsoft Excel sheets to analyze genotypic data. Available at http://www.ib.usp.br/~lgea/fofpop. Accessed 11 March 2011
Francisco FO, Brito RM, Santiago LR, Gonçalves PHP, Pioker FC, Domingues-Yamada AMT, Arias MC (2011) Isolation and characterization of 15 microsatellite loci in the stingless bee Plebeia remota (Apidae: Meliponini). Conserv Genet Resour 3:417–419. doi:10.1007/s12686-010-9369-0
Freitas BM, Oliveira-Filho JH (2001) Criação racional de mamangavas: para polinização em áreas agrícolas. Banco do Nordeste, Fortaleza
Freitas BM, Oliveira-Filho JH (2003) Ninhos racionais para mamangava (Xylocopa frontalis) na polinização do maracujá-amarelo (Passiflora edulis). Cienc Rural [online] 33:1135–1139
Gerling D, Velthuis HHW, Hefetz A (1989) Bionomics of the large carpenter bees of the genus Xylocopa. Ann Rev Entomol 34:163–190
Hogendoorn K (1996) Socio-economics of brood destruction during supersedure in the carpenter bee Xylocopa pubescens. J Evol Biol 9:931–952
Hogendoorn K, Velthuis HHW (1993) The sociality of Xylocopa pubescens: does a helper really help? Behav Ecol Sociobiol 32:247–257
Hurd PD (1978) An annotated catalog of the carpenter bees (genus Xylocopa Latr.) of the Western Hemisphere (Hymenoptera: Anthophoridae). Smithsonian Institution Press, Washington
Pereira M, Garófalo CA (2010) Biologia da nidificação de Xylocopa frontalis e Xylocopa grisescens (Hymenoptera, Apidae, Xylocopini) em ninhos-armadilha. Oecol Aust 14:193–209
Rousset F (2008) GENEPOP’007: a complete re-implementation of the GENEPOP software for Windows and Linux. Mol Ecol Resour 8:103–106
Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, pp 365–386
Stark RE (1992) Cooperative nesting in the multivoltine large carpenter bee Xylocopa sulcatipes Maa (Apoidea: Anthophoridae): do helpers gain or lose to solitary females? Ethology 91:301–310
Stark RE, Hefetz A, Gerling D, Velthuis HHW (1990) Reproductive competition involving oophagy in the socially nesting bee Xylocopa sulcatipes. Naturwissenschaften 77:38–40
Acknowledgments
This research was financially supported by the Coordenadoria de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) through Programa Nacional de Cooperação Acadêmica (PROCAD), Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP-Proc. 10/50597-5) and Fundação de Amparo à Pesquisa de Minas Gerais (FAPEMIG). We are grateful to Paulo Eugênio Oliveira for coordination of the Procad Project No. 158/2007. Solange Cristina Augusto received a post-doctoral fellowship from CAPES.
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Augusto, S.C., Gonçalves, P.H.P., de Oliveira Francisco, F. et al. Microsatellite loci for the carpenter bee Xylocopa frontalis (Apidae, Xylocopini). Conservation Genet Resour 4, 315–317 (2012). https://doi.org/10.1007/s12686-011-9536-y
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DOI: https://doi.org/10.1007/s12686-011-9536-y