The development of 20 microsatellite loci for the Australian marine mollusk, Donax deltoides, through next generation DNA sequencing
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Next Generation DNA sequencing was used to develop a suite of microsatellite markers for the marine mollusk, Donax deltoides. A total of 20 polymorphic loci were identified and 12 characterized using 30 individuals from a single population (Venus Bay) in south eastern Australia. We observed moderate to high genetic variation across most loci (mean number of alleles per locus = 7.3; mean heterozygosity = 0.633) with only a single locus (Ddel32) displaying significant deviation from Hardy–Weinberg equilibrium. Marker independence was confirmed with tests for linkage disequilibrium, however two loci were found to be influenced by null alleles. The 10 viable markers characterized in the present study provide a valuable resource for future population genetic assessments and fisheries management of D. deltoides in Australia.
KeywordsMicrosatellite development Next generation DNA sequencing Donax deltoides Marine mollusk
The authors would like to acknowledge Deakin University for financial support through the Environmental Sustainability Faculty Research Cluster (ESFRC). This project was also assisted by the Victorian Government through revenue from Recreational Fishing Licenses.
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