Conservation Genetics Resources

, Volume 4, Issue 2, pp 213–216 | Cite as

Characterisation of polymorphic microsatellite loci in Hephaestus fuliginosus and cross-amplification in closely related Hephaestus tulliensis

  • Brian Wade JamandreEmail author
  • Kathryn Real
  • Jane Hughes
Technical Note


Eleven polymorphic microsatellite loci were isolated and characterised from sooty grunter (Hephaestus fuliginosus) and Tully grunter (H. tulliensis) from Northern Australia. Each primer pair amplified good quality and polymorphic products. The versatilities of these markers were tested using different H. fuliginosus populations and cross-amplified to a closely related species, H. tulliensis, found within the northern Australian wet tropics. The number of alleles ranged from 5 to 20 per locus and expected heterozygosities ranged from 0.043 to 0.926. All loci conformed to Hardy–Weinberg expectations, with the exception of 2Hf44 and 2Hf53 for Daly R. and Mitchell R. populations of H. fuliginosus, respectively. No evidence of linkage disequilibrium was detected between any pair of loci. The markers reported here would be very useful for population genetic studies, evolution and conservation of these species.


Population genetic analysis Northern Australia Freshwater fish Grunters 



This work was supported by Tropical Rivers and Coastal Knowledge Commonwealth Environmental Research Facility (TRaCK) that receives major funding through Australian Government’s Commonwealth Environment Research facilities initiative, Australian Government’s Raising National Water Standards Program, Land and Water Australia, Fisheries Research and Development Corporation, and Queensland Government’s Smart State Innovation Fund. We thank Courtenay Mills and Andrew Bentley for providing the samples. Brian Wade Jamandre is supported by the Endeavour Research Awards of the Australian Government’s Department of Education, Employment and Work Relations.


  1. Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15Google Scholar
  2. Excoffier L, Lischer HE (2010) Arlequin suite ver. 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Mol Ecol Res 10:564–567CrossRefGoogle Scholar
  3. Glenn TC, Schable NA (2005) Isolating microsatellite DNA loci. Methods Enzymol 395:202–222PubMedCrossRefGoogle Scholar
  4. Oosterhout C, Hutchinson W, Wills D, Shipley P (2004) MICROCHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538CrossRefGoogle Scholar
  5. Pusey B, Kennard M, Arthington A (2004) Freshwater fishes of north-eastern Australia. CSIRO, MelbourneGoogle Scholar
  6. Raymond M, Rousset F (1995) GENEPOP (Version 1.2): a population genetics software for exact tests and ecumenicism. J Hered 86(24):8–249Google Scholar
  7. Real K, Schmidt D, Hughes J (2009) Mogurnda adspersa microsatellite markers: multiplexing and multi-tailed primer tagging. Conserv Genet Resour 1:411–414CrossRefGoogle Scholar
  8. Schuelke M (2000) An economic method for the fluorescent labeling of PCR fragments. Nat Biotechnol 18:233–234PubMedCrossRefGoogle Scholar
  9. Scribner KT, Page KS, Bartron ML (2001) Hybridization in freshwater fishes: a review of case studies and cytonuclear methods of biological inference. Rev Fish Biol Fish 10:293–323CrossRefGoogle Scholar
  10. Vallone PM, Butler JM (2004) AutoDimer: a screening tool for primer-dimer and hairpin structures. Biotechniques 372:226–231Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Brian Wade Jamandre
    • 1
    Email author
  • Kathryn Real
    • 1
  • Jane Hughes
    • 1
  1. 1.Australian Rivers InstituteGriffith UniversityNathanAustralia

Personalised recommendations