Cross-species amplification and development of microsatellites for six species of European Coenagrionid damselflies
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We describe the cross-amplification and development of new loci for six species of closely related European damselflies. First, twenty-nine published microsatellites for the damselflies Coenagrion puella and C. mercuriale were multiplexed using M13-tagged primers, tested on 23 individuals, and then cross-species amplified on 21–26 individuals of C. armatum, C. johanssoni, C. pulchellum and C. scitulum. Second, sixteen new primers were developed for use in C. armatum, C. johanssoni and C. scitulum, and screened on 21 individuals. Values for observed heterozygosities and number of alleles ranged between 0.00–0.87 and 2–19 respectively (over all loci and species). For all species the tested loci provide a minimum of 1–8 usable markers for population genetic studies.
KeywordsCoenagrion Damselfly Microsatellite Cross-species amplification
This work was funded by Stiftelsen J Gust Richert, Carl Tryggers Foundation and FORMAS. We thank Kenyon Mobley, Maria Lindgren, Owen Rowe and Nathaniel Street for their assistance.
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