Conservation Genetics Resources

, Volume 4, Issue 1, pp 191–196 | Cite as

Cross-species amplification and development of microsatellites for six species of European Coenagrionid damselflies

  • Helena Johansson
  • Pär K. Ingvarsson
  • Frank Johansson
Technical Note

Abstract

We describe the cross-amplification and development of new loci for six species of closely related European damselflies. First, twenty-nine published microsatellites for the damselflies Coenagrion puella and C. mercuriale were multiplexed using M13-tagged primers, tested on 23 individuals, and then cross-species amplified on 21–26 individuals of C. armatum, C. johanssoni, C. pulchellum and C. scitulum. Second, sixteen new primers were developed for use in C. armatum, C. johanssoni and C. scitulum, and screened on 21 individuals. Values for observed heterozygosities and number of alleles ranged between 0.00–0.87 and 2–19 respectively (over all loci and species). For all species the tested loci provide a minimum of 1–8 usable markers for population genetic studies.

Keywords

Coenagrion Damselfly Microsatellite Cross-species amplification 

Notes

Acknowledgments

This work was funded by Stiftelsen J Gust Richert, Carl Tryggers Foundation and FORMAS. We thank Kenyon Mobley, Maria Lindgren, Owen Rowe and Nathaniel Street for their assistance.

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Helena Johansson
    • 1
  • Pär K. Ingvarsson
    • 2
    • 3
  • Frank Johansson
    • 4
  1. 1.Department of BiosciencesHelsinki UniversityHelsinkiFinland
  2. 2.Department of Ecology and Environmental ScienceUmeå UniversityUmeåSweden
  3. 3.Umeå Plant Science CentreUmeå UniversityUmeåSweden
  4. 4.Department of Ecology and GeneticsUppsala UniversityUppsalaSweden

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