Cross-species amplification and development of microsatellites for six species of European Coenagrionid damselflies
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We describe the cross-amplification and development of new loci for six species of closely related European damselflies. First, twenty-nine published microsatellites for the damselflies Coenagrion puella and C. mercuriale were multiplexed using M13-tagged primers, tested on 23 individuals, and then cross-species amplified on 21–26 individuals of C. armatum, C. johanssoni, C. pulchellum and C. scitulum. Second, sixteen new primers were developed for use in C. armatum, C. johanssoni and C. scitulum, and screened on 21 individuals. Values for observed heterozygosities and number of alleles ranged between 0.00–0.87 and 2–19 respectively (over all loci and species). For all species the tested loci provide a minimum of 1–8 usable markers for population genetic studies.
KeywordsCoenagrion Damselfly Microsatellite Cross-species amplification
This work was funded by Stiftelsen J Gust Richert, Carl Tryggers Foundation and FORMAS. We thank Kenyon Mobley, Maria Lindgren, Owen Rowe and Nathaniel Street for their assistance.
- Holleley CE, Geerts PG (2009) Multiplex manager 1.0: a crossplatform computer program that plans and optimizes multiplex PCR. BioTechniques 46:511–517Google Scholar
- Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods protocols: methods in molecular biology. Humana Press, TotowaGoogle Scholar
- Sahlen G, Bernard R, Cordero Rivera A, Ketelaar R, Suhling F (2006) Critical species of Odonata in Europe. Int J Odonatol 7:385–398Google Scholar
- Schneider S, Roessli D, Excoffier L (2000) Arlequin: a software for population genetics data analysis. V3.01. Genetics and Biometry Lab, Dept. of Anthropology, University of GenevaGoogle Scholar
- Watts PC, Thompson DH, Kemp SJ (2004b) Cross-species amplification of microsatellite loci in some European zygopteran species (Odonata: Coenagrionidae). Int J Odonatol 7:87–96Google Scholar