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Conservation Genetics Resources

, Volume 4, Issue 1, pp 113–116 | Cite as

Isolation and characterisation of novel microsatellite and mitochondrial DNA markers for the Eastern Water Dragon (Physignathus lesueurii)

  • C. H. FrèreEmail author
  • P. J. Prentis
  • T. Ezaz
  • A. Georges
Technical Note

Abstract

Habitat fragmentation as a result of urbanisation is a growing problem for native lizard species. The Eastern Water Dragon (Physignathus lesueurii) is a social arboreal agamid lizard, native to Australia. This species represents an ideal model species to investigate the effect of urbanisation because of their prominent abundance in the urban landscape. Here we describe the isolation and characterisation of a novel set of 74 di-, tri-, and tetra-microsatellites from which 18 were selected and optimised into two multiplexes. The 18 microsatellites generated a total 148 alleles across the two populations. The number of alleles per locus varied from 2 to 18 alleles and measures of Ho and He varied from 0.395 to 0.877 and from 0.441 to 0.880, respectively. We also present primers for four novel mitochondrial DNA (mtDNA) markers. The combined length of the four mtDNA marker pairs was 2,528 bp which included 15 nucleotides changes. In comparison to threatened species, which are generally characterised by small population sizes, the Eastern Water Dragon represents an ideal model species to investigate the effect of urbanisation on their behavioural ecology and connectivity patterns among populations.

Keywords

Paternity Agamid lizards Agamidae Gene flow Kinship 454 Pryosequencing 

Notes

Acknowledgments

The research was carried out under permit no. WISP08423810 issued by Environment Protection Agency. Ethics approval was given from The University of Queensland (no. GPA/378/10). This work is partially supported by Australian Research Council Discovery Grants (DP110104377) awarded to A.G and T.E.

Supplementary material

12686_2011_9487_MOESM1_ESM.doc (223 kb)
Supplementary material 1 (DOC 223 kb)

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • C. H. Frère
    • 1
    Email author
  • P. J. Prentis
    • 2
  • T. Ezaz
    • 3
  • A. Georges
    • 3
  1. 1.School of Biological SciencesUniversity of QueenslandBrisbaneAustralia
  2. 2.Biogeosciences DisciplineQueensland University of TechnologyBrisbaneAustralia
  3. 3.Institute for Applied EcologyUniversity of CanberraCanberraAustralia

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