Development and characterization of 24 polymorphic microsatellite loci in two Antirrhinum majus subspecies (Plantaginaceae) using pyrosequencing technology
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The recently described procedure of microsatellite-enriched libraries pyrosequencing was used to isolate 557 microsatellite loci in two subspecies of the snapdragon Antirrhinum majus (Plantaginaceae). Four multiplex PCR sets were optimized to genotype 24 polymorphic markers. The number of alleles per locus ranged from one to 19 and observed heterozygosity varied from zero to 0.95 in four Pyrenean populations. Cross-species amplification of these loci was successfully demonstrated in additional Antirrhinum species. This set of microsatellite markers will be useful for studying population genetics, plant-pollinators interactions and inheritance of developmental genes in A.majus.
KeywordsAntirrhinum Microsatellites Plantaginaceae Pyrenees Pyrosequencing
- Khimoun A, Burrus M, Andalo C, Liu ZL, Vicédo-Cazettes C, Thébaud C, Pujol B (2011) Locally asymmetric introgressions between subspecies suggest circular range expansion at the Antirrhinum majus global scale. J Evol Biol. doi: 10.1111/j.1420-9101.2011.02276.x
- Malausa T, Gilles A, Meglécz E, Blanquart H, Duthoy S, Costedoat C, Dubut V, Pech N, Castagnone-Sereno P, Délye C, Feau N, Frey P, Gauthier P, Guillemaud T, Hazard L, Le Corre V, Lung-Escarmant B, Malé P-JG, Ferreira S, Martin J-F (2011) High throughput microsatellites isolation with 454 GS-FLX Titanium pyrosequencing. Mol Ecol Resour. doi: 10.1111/j.1755-0998.2011.02992.x
- Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact test and ecumenism. J Hered 86:248–249Google Scholar