Conservation Genetics Resources

, Volume 4, Issue 1, pp 75–79 | Cite as

Development and characterization of 24 polymorphic microsatellite loci in two Antirrhinum majus subspecies (Plantaginaceae) using pyrosequencing technology

  • Gabriel D. G. Debout
  • Emeline Lhuillier
  • Pierre-Jean G. Malé
  • Benoît Pujol
  • Christophe Thébaud
Technical Note

Abstract

The recently described procedure of microsatellite-enriched libraries pyrosequencing was used to isolate 557 microsatellite loci in two subspecies of the snapdragon Antirrhinum majus (Plantaginaceae). Four multiplex PCR sets were optimized to genotype 24 polymorphic markers. The number of alleles per locus ranged from one to 19 and observed heterozygosity varied from zero to 0.95 in four Pyrenean populations. Cross-species amplification of these loci was successfully demonstrated in additional Antirrhinum species. This set of microsatellite markers will be useful for studying population genetics, plant-pollinators interactions and inheritance of developmental genes in A.majus.

Keywords

Antirrhinum Microsatellites Plantaginaceae Pyrenees Pyrosequencing 

Supplementary material

12686_2011_9478_MOESM1_ESM.doc (83 kb)
Supplementary material 1 (DOC 83 kb)

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Gabriel D. G. Debout
    • 1
    • 2
  • Emeline Lhuillier
    • 1
    • 2
    • 3
  • Pierre-Jean G. Malé
    • 1
    • 2
  • Benoît Pujol
    • 1
    • 2
  • Christophe Thébaud
    • 1
    • 2
  1. 1.UPS, EDB (Laboratoire Evolution et Diversité Biologique)Université de ToulouseToulouseFrance
  2. 2.CNRS, EDB (Laboratoire Evolution et Diversité Biologique)ToulouseFrance
  3. 3.Genopole Toulouse Midi-Pyrénées, Plateforme GénomiqueAuzevilleFrance

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