Development and characterization of 24 polymorphic microsatellite loci in two Antirrhinum majus subspecies (Plantaginaceae) using pyrosequencing technology
The recently described procedure of microsatellite-enriched libraries pyrosequencing was used to isolate 557 microsatellite loci in two subspecies of the snapdragon Antirrhinum majus (Plantaginaceae). Four multiplex PCR sets were optimized to genotype 24 polymorphic markers. The number of alleles per locus ranged from one to 19 and observed heterozygosity varied from zero to 0.95 in four Pyrenean populations. Cross-species amplification of these loci was successfully demonstrated in additional Antirrhinum species. This set of microsatellite markers will be useful for studying population genetics, plant-pollinators interactions and inheritance of developmental genes in A. majus.
KeywordsAntirrhinum Microsatellites Plantaginaceae Pyrenees Pyrosequencing
We are grateful to M. Burrus and C. Andalo for providing us with samples of Antirrhinum other than A. majus. Support for this study was provided by a research grant BQR UFR SVT from the University of Toulouse Paul Sabatier awarded to BP. GDGD and P-JGM were supported by ATER fellowship grants from the University Paul Sabatier. This article is dedicated to the memory of Gabriel Debout who started this study but disappeared in a tragic accident before the completion of this work.
- Khimoun A, Burrus M, Andalo C, Liu ZL, Vicédo-Cazettes C, Thébaud C, Pujol B (2011) Locally asymmetric introgressions between subspecies suggest circular range expansion at the Antirrhinum majus global scale. J Evol Biol. doi: 10.1111/j.1420-9101.2011.02276.x
- Malausa T, Gilles A, Meglécz E, Blanquart H, Duthoy S, Costedoat C, Dubut V, Pech N, Castagnone-Sereno P, Délye C, Feau N, Frey P, Gauthier P, Guillemaud T, Hazard L, Le Corre V, Lung-Escarmant B, Malé P-JG, Ferreira S, Martin J-F (2011) High throughput microsatellites isolation with 454 GS-FLX Titanium pyrosequencing. Mol Ecol Resour. doi: 10.1111/j.1755-0998.2011.02992.x
- Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact test and ecumenism. J Hered 86:248–249Google Scholar