Development of tetranucleotide microsatellite loci and a non-invasive DNA sampling method for Texas horned lizards (Phrynosoma cornutum)
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We developed a non-invasive DNA sampling method and 15 tetranucleotide microsatellite markers for Texas horned lizards (Phrynosoma cornutum). Swabbing the cloaca with a small cotton swab and preserving the cells in lysis buffer was an effective method to obtain tissue for DNA extraction. Loci were highly polymorphic with 8–25 alleles and observed heterozygosity was high (0.71–0.96). Some of these loci can also be used for round-tailed horned lizards (P. modestum).
KeywordsMicrosatellites Phrynosoma cornutum Horned lizards Dispersal Parentage Non-invasive sampling
This work was supported by a Wildlife Diversity Conservation Grant from Texas Parks and Wildlife Department (TPWD) and an Andrews Institute Research Award, TCU to DAW and AMH. We thank Gad Perry for suggesting the use of cloacal swabs, and Chip Ruthven, members of the Horned Lizard Conservation Society, and TPWD personnel for collecting samples. Research was conducted with a permit from TPWD and IACUC approval from TCU.
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