New microsatellite markers for the assessment of fine-scale dispersal patterns in the endangered montane caddisfly Drusus discolor
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We present a panel of variable microsatellite markers for the montane caddisfly Drusus discolor, a rare species that we use as a model for the assessment of climate change effects on the montane freshwater community. 454 sequencing technology was used in order to generate random sequence data. Of a total of 2,648 microsatellite-carrying fragments identified, 11 variable, reproducible markers were generated and tested on 41 individuals from two populations. Allelic diversity ranged from 3 to 10 alleles and heterozygosity values varied from 0 to 0.89 (H O) and 0.12 to 0.86 (H E). A cross-species test on four caddisfly species showed that a subset of markers amplifies in related taxa.
KeywordsShort tandem repeats Landscape genetics Dispersal Aquatic insects Microsatellite development Montane biodiversity Global climate change
We thank Steffen U. Pauls (Biodiversity and Climate Research Center) for providing samples for the cross-species test. Funding comes from the Deutsche Forschungsgemeinschaft (HA3431/4-1). Additional funding is provided by the research funding program Landes-Offensive zur Entwicklung Wissenschaftlich-ökonomischer Exzellenz (LOEWE) of Hesse’s Ministry of Higher Education, Research, and the Arts.
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