Microsatellite loci for the endangered growling grass frog (Litoria raniformis), with cross amplification in other Australian frog species
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The growling grass frog, Litoria raniformis, was once abundant in south-eastern Australia but has suffered severe declines over the past 20 years. Here we describe nine polymorphic microsatellite loci for L. raniformis. Genetic variation was assessed in 59 individuals from Somerton, Victoria in south-eastern Australia. The number of alleles per locus ranged from three to 16. Cross amplification was assessed in ten other Australian hylid frogs. These microsatellite markers will be used to investigate impacts of urbanization on dispersal and gene flow in L. raniformis.
KeywordsLitoria raniformis Microsatellites Australia Bell frog Hylidae
We thank J. Vörös for providing samples for cross-amplification, D. Chapple for comments on manuscript drafts and the Australian Genome Research Facility for assistance in the microsatellite library development and genotyping loci. Fieldwork was completed in accordance with Animal Ethics guidelines (La Trobe University AEC02/24(L)/V4), with collection permits (Victorian Department of Sustainability and Environment; Permit 10003005). Research funding provided by Growling Grass Frog Trust Fund (to GWH), Victorian Department of Sustainability and Environment (to GWH) and the Australian Research Council (to JM and JA, Project Number LP0667815).
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