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Conservation Genetics Resources

, Volume 3, Issue 3, pp 565–567 | Cite as

Isolation and characterization of 21 polymorphic microsatellite loci in the Japanese dace (Tribolodon hakonensis)

  • Noriyuki KoizumiEmail author
  • Thomas W. Quinn
  • Myeongsoo Park
  • Jennifer Fike
  • Kazuya Nishida
  • Takeshi Takemura
  • Keiji Watabe
  • Atsushi Mori
Technical Note
  • 142 Downloads

Abstract

Twenty one polymorphic microsatellite loci for the Japanese dace (Tribolodon hakonensis) were isolated and characterized. The number of observed alleles per locus in 32 individuals ranged from 3 to 30. The observed and expected heterozygosities ranged from 0.125 to 0.969 and from 0.175 to 0.973, respectively. All loci conformed to Hardy–Weinberg equilibrium, no linkage disequilibrium was observed between pairs of loci and no loci showed evidence of null alleles. These microsatellite loci will be useful for investigating the intraspecific genetic variation and population structure of this species.

Keywords

Cyprinidae Microsatellite primers Genetic diversity Conservation Rural ecosystem 

Notes

Acknowledgments

This study was supported in part by a Grant-in-Aid for Scientific Research (C-20580270, C-21780228, C-22580281 and B-22380133) from the Japan Society for the Promotion of Science. The first author would like to thank Mses. K. Yamanoi and P. Goto for analysis support.

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Noriyuki Koizumi
    • 1
    Email author
  • Thomas W. Quinn
    • 2
  • Myeongsoo Park
    • 3
  • Jennifer Fike
    • 4
  • Kazuya Nishida
    • 1
  • Takeshi Takemura
    • 1
  • Keiji Watabe
    • 1
  • Atsushi Mori
    • 1
  1. 1.National Institute for Rural EngineeringTsukuba, IbarakiJapan
  2. 2.Department of Biological SciencesUniversity of DenverDenverUSA
  3. 3.Rural Research InstituteAnsan, GyeonggiKorea
  4. 4.U.S. Geological Survey, Fort Collins Science CenterFort CollinsUSA

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