Development and characterization of nine microsatellite loci for the endangered Kidneyshell, Ptychobranchus fasciolaris, and cross-amplification in closely-related lampsilines (Bivalvia: Unionoida)
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Nine polymorphic microsatellite loci were isolated and characterized for the unionid Ptychobranchus fasciolaris. Screening of individuals from two Ontario rivers detected 4–15 alleles per locus and within-population heterozygosities from 0.280 to 0.800. Cross-amplification tests in six other lampsiline species largely yielded polymorphic data, suggesting that these loci should prove useful in population assessments for other species as well.
KeywordsUnionid Kidneyshell mussel Ontario Microsatellite Population genetics
Funding was provided by the ESRF of World Wildlife Fund Canada, Fisheries and Oceans Canada, and Ontario Ministry of Natural Resources. Friedrich Fischer, Ryan Hill, and Kirsty Hill assisted with field sampling. Missouri State University, University of Alabama Museum and Auburn University Museum provided tissue samples.
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