Development and characterization of 10 polymorphic microsatellite loci for the blue shark, Prionace glauca, and their cross shark-species amplification
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Ten polymorphic nuclear microsatellite loci were developed from a microsatellite enriched genomic library of the blue shark, Prionace glauca. The utility of these markers for genetic studies of this globally distributed, heavily exploited, oceanic predator was assessed by screening 120 specimens sampled from six locations throughout the species’ range. Both moderately and highly polymorphic marker loci were identified. Three to 35 alleles were found to be segregating per locus (mean 10.1) with observed heterozygosities ranging from 24 to 91%. Evaluation of the cross-species amplification of these markers across 18 additional shark species indicates that these microsatellites are potentially useful for genetic studies of other species of conservation concern.
KeywordsBlue shark Prionace glauca Carcharhinus Polymorphic Microsatellite Cross-species
S. Fitzpatrick was supported by a DEL PhD studentship. We thank N. McKeown for assistance with lab work. This work was supported by the Beaufort Fish Population Genetics Award under the Sea Change Strategy and the Strategy for Science Technology and Innovation, funded under the Marine Research Sub-Programme of the National Development Plan 2007–2013 (Prodöhl lab), and the Save Our Seas Foundation and Swiss Shark Foundation (Shivji lab).
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