Conservation Genetics Resources

, Volume 3, Issue 3, pp 449–456 | Cite as

Twenty-nine microsatellite markers for two Australian freshwater turtles, Elseya albagula and Emydura macquarii krefftii: development from 454-sequence data and utility in related taxa

Technical Note

Abstract

454 shotgun sequencing was used to identify thousands of in silico microsatellite loci from two Australian freshwater turtle species (Pleurodira, Chelidae), Elseyaalbagula and Emyduramacquariikrefftii. Twenty-nine polymorphic microsatellites were subsequently developed and tested as markers, 15 for E. m. krefftii and 14 for E. albagula. The former marker set showed greater allelic richness (mean NA = 7.7, range 3–15) than the latter (mean NA = 4.4, range 2–8). Levels of expected heterozygosity were high for both marker suites (mean HE = 0.718, range 0.177–0.875; and mean HE = 0.567, range 0.317–0.827, respectively), indicating both are suitable for studies of population genetic structure, connectivity and mating systems in these taxa. Cross-species amplification in 12 other short-necked freshwater turtle taxa highlighted the potential utility of these markers in other species, notably Emydura spp., Rheodytes leukops and Elusor macrurus.

Keywords

Testudines Chelidae 454 pyrosequencing Multiplex PCR Gene flow River regulation 

Notes

Acknowledgments

We thank Queensland Turtle Research and Jason Schaffer for providing tissue samples and fieldwork opportunities. Arthur Georges kindly provided many tissue samples for cross-species testing. Kyall Zenger provided helpful sequencing advice and assistance. Ivan Lawler, Dave Jones and Carolyn Smith-Keune also provided invaluable advice and support. This work was partially funded by research grants from the Wildlife Preservation Society of Queensland, Wildlife Preservation Society of Australia, and the Peter Rankin Trust Fund for Herpetology. Research was conducted under ethics permit no. A1444.

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Erica Todd
    • 1
  • David Blair
    • 1
  • Mark Hamann
    • 2
  • Dean Jerry
    • 1
  1. 1.School of Marine and Tropical BiologyJames Cook UniversityTownsvilleAustralia
  2. 2.School of Earth and Environmental SciencesJames Cook UniversityTownsvilleAustralia

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