Isolation and characterization of novel microsatellite loci in Vaccinium arctostaphylos L
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Abstract
For the first time, this study has described the characterization of 25 novel polymorphic microsatellite loci from a repeat-enriched genomic library of V. arctostaphylos using a modified FIASCO method. Polymorphism of each locus was assessed in 64 individuals of the Vaccinium. The average allele number of the microsatellites, observed (HO) and expected (HE) heterozygosities were 5.88, 0.85 and 0.67 per locus, respectively. The polymorphic information content (PIC) value and inbreeding coefficient (F) ranged from 0.26 to 0.87 and −0.72 to 0.03, respectively. Ten of the twenty-five loci showed significant departures from Hardy–Weinberg equilibrium. These microsatellite loci could be useful to study genetic diversity and population structure of V.arctostaphylos.
Keywords
Microsatellite marker FIASCO Genetic diversity Vaccinium arctostaphylos LNotes
Acknowledgments
We gratefully thank Ms. Rooshanak Sepehrifar for providing us with the plant materials. This study was supported by Agricultural Biotechnology Research Institute of Iran (ABRII).
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