Polymorphic microsatellite DNA markers for the Amazonian catfish Pseudoplatystoma punctifer (Siluriformes: Pimelodidae)
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The large catfish Pseudoplatystoma punctifer has a high commercial value in the Amazon. Fifteen polymorphic microsatellite loci were isolated and characterized in 36–39 individuals of this species sampled at the Purus river (Amazon Basin). The number of alleles per locus ranged from 2 to 18 (average 7.4), the observed heterozygosity (HO) from 0.025 to 0.972 and the expected heterozygosity (HE) from 0.025 to 0.931. Two loci showed a Hardy–Weinberg Equilibrium deviation. No linkage disequilibrium was found among the studied loci. Fourteen microsatellite loci were cross-amplified successfully in six other Pimelodidae species. Therefore, these loci may be valuable for other studies of population genetic structure as well as for catfish conservation in the Amazon basin.
KeywordsSurubim Microsatellite loci Cross-amplification Amazon biodiversity Conservation Siluriformes
This work was supported by CNPq/CT-Amazônia (554054/2006–2010) to J.S.B, FAPEAM/PIPT (514/2008) to J.A.A.G. and CNPq/CT-Amazônia (575603/2008–2009) to IPF and PROCAD-Amazônia-INPA/UNICAMP/UFRGS/CAPES (023/2006) to MSR. IBAMA granted the Permissions (Nºs 130 and 14278-1) to collect tissue samples. The authors thank Anete Pereira de Souza (CBMEG/UNICAMP) for crucial lab assistance, Giselle Guimarães and Luciana Batista for support in the laboratory, I. B. Francini for manuscript review, Vera Val (LEEM/INPA) for making the sequencing on the ABI 3130 available and Claudio Oliveira (UNESP/Botucatu) for P. corruscans samples. A.S.C.M. was supported by PIBIC/CNPq/INPA fellowship.
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