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Conservation Genetics Resources

, Volume 3, Issue 1, pp 177–180 | Cite as

First polymorphic microsatellites for the gyrodactylids (Monogenea), an important group of fish pathogens

  • Patrícia J. Faria
  • Colin M. Lazarus
  • Cock van Oosterhout
  • Phil D. Harris
  • Joanne CableEmail author
Technical Note

Abstract

We describe the isolation of microsatellites from three monogenean flatworms (Gyrodactylus turnbulli, G. bullatarudis and G. salaris) which have utility for evolutionary studies and pathogen diagnosis. Six polymorphic loci (1–5 alleles each) were isolated from G. turnbulli and seven from G. bullatarudis; observed heterozygosity ranged from 0.05 to 0.46 and from 0.1 to 0.5, respectively. Only monomorphic loci were isolated in G. salaris. These microsatellites will be useful for determining the mechanism of gyrodactylid reproduction and understanding the role of these important pathogens in the evolutionary history of their fish hosts.

Keywords

Gyrodactylid monogeneans Microsatellites 

Notes

Acknowledgments

Prof. Tor Bakke provided G. salaris, Anne Harasta and Marie Le Goff Vitry gave technical support. Project funded by the European Community Framework Programme 6 (MTKD-CT-2005-030018).

References

  1. Bakke TA, Harris PD, Cable J (2002) Host specificity dynamics: observation on gyrodactylid monogeneans. Int J Parasitol 32:281–308CrossRefPubMedGoogle Scholar
  2. Bakke TA, Cable J, Harris PD (2007) The biology of gyrodactylid monogeneans: the “Russian-doll killers”. Adv Parasitol 64:162–376Google Scholar
  3. Cable J (2010) Poeciliid parasites. In: Evans JP, Pilastro A, Schlupp I (eds) Ecology & evolution of Poeciliid fishes. Chicago University Press (in press)Google Scholar
  4. Cable J, Harris PD (2002) Gyrodactylid developmental biology: historical review, current status and future trends. Int J Parasitol 32:255–280CrossRefPubMedGoogle Scholar
  5. Excoffier L, Laval G, Schneider S (2005) Arlequin ver. 3.0: an integrated software package for population genetics data analysis. Evol Bioinform Online 1:47–50PubMedGoogle Scholar
  6. Glenn TC, Schable NA (2005) Isolating microsatellite DNA loci. Meth Enzymol 395:202–222CrossRefPubMedGoogle Scholar
  7. Harris PD, Shinn AP, Cable J, Bakke TA (2004) Nominal species of the genus Gyrodactylus v. Nordmann 1822 (Monogenea: Gyrodactylidae), with a list of principal host species. Syst Parasitol 59:1–27CrossRefPubMedGoogle Scholar
  8. Harris PD, Shinn AP, Cable J, Bakke TA, Bron J (2008) GyroDb: gyrodactylid monogeneans on the web. Trends Parasitol 24:109–111CrossRefPubMedGoogle Scholar
  9. Jones KC, Levine KF, Banks JD (2002) Characterization of 11 polymorphic tetranucleotide microsatellites for forensic applications in California elk (Cervus elaphus canadensis). Mol Ecol Notes 2:425–427CrossRefGoogle Scholar
  10. Kennedy CR (1994) The ecology of introductions. In: Pike AW, Lewis JW (eds) Parasitic diseases of fish. Samara Publishing Ltd, Tresaith, pp 189–208Google Scholar
  11. King TA, Cable J (2007) Experimental infections of the monogenean Gyrodactylus turnbulli indicate that it is not a strict specialist. Int J Parasitol 37:663–672CrossRefPubMedGoogle Scholar
  12. Rozen S, Skaletsky HJ (2000) Primer 3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, pp 365–386Google Scholar
  13. Taylor JR (1997) An introduction to error analysis: the study of uncertainties in physical measurements. University Science Books, SausalitoGoogle Scholar
  14. van Oosterhout C, Weetman D, Hutchinson WF (2006) Estimation and adjustment of microsatellite null alleles in nonequilibrium populations. Mol Ecol Notes 6:255–256CrossRefGoogle Scholar
  15. van Oosterhout C, Mohammed RS, Hansen H, Archard GA, McMullan M, Weese DJ, Cable J (2007a) Selection by parasites in spate conditions in wild Trinidadian guppies (Poecilia reticulata). Int J Parasitol 37:805–812CrossRefPubMedGoogle Scholar
  16. van Oosterhout C, Smith AM, Hänfling B, Ramnarine IW, Mohammed RS, Cable J (2007b) The guppy as a conservation model: implications of parasitism and inbreeding for reintroduction success. Conserv Biol 21:1573–1583PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Patrícia J. Faria
    • 1
  • Colin M. Lazarus
    • 2
  • Cock van Oosterhout
    • 3
  • Phil D. Harris
    • 4
  • Joanne Cable
    • 1
    Email author
  1. 1.School of BiosciencesCardiff UniversityCardiffUK
  2. 2.School of Biological SciencesUniversity of BristolBristolUK
  3. 3.Department of Biological SciencesUniversity of HullHullUK
  4. 4.National Centre for Biosystematics, Natural History MuseumUniversity of OsloOsloNorway

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