Twelve microsatellite markers in the invasive tunicate, Didemnum vexillum, isolated from low genome coverage 454 pyrosequencing reads
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454 pyrosequencing reads were used to isolate microsatellites in the global marine ascidian invader, Didemnum vexillum. This method allows simple and cost-effective isolation of new markers from organisms without existing genomic information and, to our knowledge, has not been used before to develop a polymorphic microsatellite marker set. Loci had between two and eleven alleles and overall mean observed and expected heterozygosities of 0.57 and 0.62, respectively. These markers will greatly facilitate research required to develop control and mitigation strategies for D. vexillum.
KeywordsDidemnidae Ascidiacea SSRs Next generation sequencing Tunicate invasions Microsatellite isolation
The authors thank Anya Epelbaum, Lucie Hannah, Ted Krickan, and Christina Simkanin for help collecting samples and to Geoff Lowe for labwork support. Matthias Herborg contributed to the early planning stages of this project. Funded by a Genomics Research and Development Initiative (Fisheries and Oceans Canada) grant to TWT.
- Brownstein MJ, Carpten JD, Smith JR (1996) Modulation of non-templated nucleotide addition by taq DNA polymerase: primer modifications that facilitate genotyping. Biotech 20:1004–1010Google Scholar
- Raymond M, Rousset F (1995) Genepop (version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86:248–249Google Scholar
- Therriault TW, Herborg L-M (2007) Risk assessment for two solitary and three colonial tunicates in both Atlantic and Pacific Canadian waters. Canadian Science Advisory Secretariat Research Document 2007/063, p 64Google Scholar