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Conservation Genetics Resources

, Volume 3, Issue 1, pp 79–81 | Cite as

Twelve microsatellite markers in the invasive tunicate, Didemnum vexillum, isolated from low genome coverage 454 pyrosequencing reads

  • Cathryn L. AbbottEmail author
  • Daniel Ebert
  • Amy Tabata
  • Thomas W. Therriault
Technical Note

Abstract

454 pyrosequencing reads were used to isolate microsatellites in the global marine ascidian invader, Didemnum vexillum. This method allows simple and cost-effective isolation of new markers from organisms without existing genomic information and, to our knowledge, has not been used before to develop a polymorphic microsatellite marker set. Loci had between two and eleven alleles and overall mean observed and expected heterozygosities of 0.57 and 0.62, respectively. These markers will greatly facilitate research required to develop control and mitigation strategies for D. vexillum.

Keywords

Didemnidae Ascidiacea SSRs Next generation sequencing Tunicate invasions Microsatellite isolation 

Notes

Acknowledgments

The authors thank Anya Epelbaum, Lucie Hannah, Ted Krickan, and Christina Simkanin for help collecting samples and to Geoff Lowe for labwork support. Matthias Herborg contributed to the early planning stages of this project. Funded by a Genomics Research and Development Initiative (Fisheries and Oceans Canada) grant to TWT.

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Copyright information

© Her Majesty the Queen in Right of Canada 2010

Authors and Affiliations

  • Cathryn L. Abbott
    • 1
    Email author
  • Daniel Ebert
    • 2
  • Amy Tabata
    • 1
  • Thomas W. Therriault
    • 1
  1. 1.Pacific Biological StationFisheries & Oceans CanadaNanaimoCanada
  2. 2.Department of BotanyUniversity of British ColumbiaVancouverCanada

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