Characterization of 13 polymorphic microsatellite loci in the European pine marten Martes martes
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A set of 13 polymorphic microsatellite markers were isolated and characterized from a genomic library enriched for dinucleotide repeats in the European pine marten Martes martes. Microsatellite loci amplification was tested on a panel of 12 tissue samples and 9 distinct hair samples collected from either road-killed or trapped animals in Tuscany, Italy. Allelic diversity was 6 and the number of alleles per locus ranged from 2 to 10. Mean observed and expected heterozygosity were 0.610 (range: 0.238–0.905) and 0.698 (range: 0.400–0.856), respectively. This novel set of microsatellite loci will be particularly useful for non-invasive genetic studies to assess population distribution and patterns of population structure and dispersal of M. martes in woodlands and fragmented habitats.
KeywordsPine marten Mustelidae Microsatellites Population genetics Non-invasive genetics
This work was funded by Ethoikos Srl. We would like to thank Gabriele Gentile for his kind collaboration and Melissa Messinese for help during field work. We are also grateful to Cataldo D’Andria for his enthusiastic support.
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