Conservation Genetics Resources

, Volume 2, Issue 1, pp 243–245 | Cite as

Nineteen polymorphic microsatellite loci isolated from the Eastern Massasauga Rattlesnake, Sistrurus c. catenatus

  • Christine S. Anderson
  • H. Lisle Gibbs
  • James Chiucchi
Technical Note

Abstract

Conservation genetic analyses can aid in the management of the eastern massasauga rattlesnake, Sistrurus c. catenatus, which is currently in decline throughout its range. To this end, we designed primers that amplify 19 novel microsatellite loci that are highly variable. Based on genotypes from 27 adults from a single population in Ohio, the number of alleles per locus ranged from two to 13 and observed and expected heterozygosities varied from 0.370 to 0.963 and 0.425 to 0.883, respectively. These loci will be useful for conservation and population genetic analyses in this endangered snake.

Keywords

Crotalidae Microsatellite DNA loci PCR primers Population genetic analyses Endangered venomous snakes 

References

  1. Avise JC (1994) Molecular markers, natural history, and evolution. Chapman and Hall, New YorkGoogle Scholar
  2. Brookfield JFY (1996) A simple new method for estimating null allele frequency from heterozygote deficiency. Mol Ecol 5:453–455PubMedGoogle Scholar
  3. DeWoody AJ, Schupp J, Kenefic L et al (2004) Universal method for producing ROX-labeled size standards suitable for automated genotyping. BioTechniques 37:348–350PubMedGoogle Scholar
  4. Faircloth BC (2008) Program note—msatcommander: detection of microsatellite repeat arrays and automated, locus-specific primer design. Mol Ecol Notes 8:92–94Google Scholar
  5. Gibbs HL, Prior KA, Weatherhead PJ, Johnson G (1997) Genetic structure of populations of the threatened eastern massasauga rattlesnake, Sistrurus c. catenatus: evidence from microsatellite DNA markers. Mol Ecol 6:1123–1132CrossRefPubMedGoogle Scholar
  6. Glenn TC, Schable NA (2005) Isolating microsatellite DNA loci. Methods Enzymol 395:202–222CrossRefPubMedGoogle Scholar
  7. Greene HW, Campbell JA (1992) The future of the pit vipers. In: Campbell JA, Brodie ED (eds) Biology of the pitvipers. Selva, Tyler, Texas, pp 421–427Google Scholar
  8. Narum SR (2006) Beyond Bonferroni: less conservative analyses for conservation genetics. Conserv Genet 7:783–787CrossRefGoogle Scholar
  9. Raymond M, Rousset F (1995) genepop (version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86:248–249Google Scholar
  10. Szymanski J (1998) Status assessment for eastern massasauga. U.S. Fish and Wildlife Service, Ft. SnellingGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Christine S. Anderson
    • 1
    • 2
  • H. Lisle Gibbs
    • 1
  • James Chiucchi
    • 1
  1. 1.Department of Evolution, Ecology and Organismal Biology, 300 Aronoff LaboratoryThe Ohio State UniversityColumbusUSA
  2. 2.Department of Biological and Environmental SciencesCapital UniversityColumbusUSA

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