Conservation Genetics Resources

, Volume 2, Issue 1, pp 243–245 | Cite as

Nineteen polymorphic microsatellite loci isolated from the Eastern Massasauga Rattlesnake, Sistrurus c. catenatus

  • Christine S. Anderson
  • H. Lisle Gibbs
  • James Chiucchi
Technical Note


Conservation genetic analyses can aid in the management of the eastern massasauga rattlesnake, Sistrurus c. catenatus, which is currently in decline throughout its range. To this end, we designed primers that amplify 19 novel microsatellite loci that are highly variable. Based on genotypes from 27 adults from a single population in Ohio, the number of alleles per locus ranged from two to 13 and observed and expected heterozygosities varied from 0.370 to 0.963 and 0.425 to 0.883, respectively. These loci will be useful for conservation and population genetic analyses in this endangered snake.


Crotalidae Microsatellite DNA loci PCR primers Population genetic analyses Endangered venomous snakes 


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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Christine S. Anderson
    • 1
    • 2
  • H. Lisle Gibbs
    • 1
  • James Chiucchi
    • 1
  1. 1.Department of Evolution, Ecology and Organismal Biology, 300 Aronoff LaboratoryThe Ohio State UniversityColumbusUSA
  2. 2.Department of Biological and Environmental SciencesCapital UniversityColumbusUSA

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