Polymorphic microsatellite markers for the overexploited Amazonian fish, Semaprochilodus insignis (Jardine and Schomburgk 1841)
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We used a microsatellite-enriched library to isolate and characterize 16 di-nucleotide microsatellite loci in Semaprochilodus insignis. Microsatellites were characterized in a population sample from Piagaçu-Purus (Purus River, Amazonas, Brazil) and in eight individuals each of S. brama from Itaipavas (Araguaia River, Pará, Brazil), S. taeniurus from Novo Airão (Negro River, Amazonas, Brazil), S. kneri (Caura River, Venezuela), and Prochilodus nigricans also from Piagaçu-Purus. In S. insignis observed heterozygosities ranged from 0.516 to 0.968, expected heterozygosities from 0.574 to 0.973, and the number of alleles per locus varied from 4 to 32 in 31 analyzed individuals. All loci were unlinked and three loci showed significant deviations from Hardy–Weinberg equilibrium. Most markers could be successfully transferred to the other four prochilodontids, making them potentially useful for conservation genetic studies of Semaprochilodus spp.
KeywordsSemaprochilodus insignis Flagtail prochilodus Population analysis Microsatellite loci Jaraqui
The development of the markers was supported by PROCAD-Amazônia—INPA/UNICAMP/UFRGS/CAPES (023/2006) grant to Miriam da Silva, CNPq/PPG7 557090/2005-9, CNPq/CT-Amazônia 553036/2005-0 and CNPq/CT-Amazônia 554057/2006-9 grants to IPF, and an NSF HRD-0931659 grant to TH. The authors thank the faculty and RAs of the PROCAD-funded course for discussion and help in the development of the markers and Stuart Willis for Venezuelan samples. This work forms a portion of KBP’s DTI fellowship from CNPq.
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