Conservation Genetics Resources

, Volume 2, Issue 1, pp 231–234 | Cite as

Polymorphic microsatellite markers for the overexploited Amazonian fish, Semaprochilodus insignis (Jardine and Schomburgk 1841)

  • Kelmer Batalha Passos
  • Adam Souza de Alencar Leão
  • Deyla Paula de Oliveira
  • Izeni Pires Farias
  • Tomas Hrbek
Technical Note

Abstract

We used a microsatellite-enriched library to isolate and characterize 16 di-nucleotide microsatellite loci in Semaprochilodus insignis. Microsatellites were characterized in a population sample from Piagaçu-Purus (Purus River, Amazonas, Brazil) and in eight individuals each of S. brama from Itaipavas (Araguaia River, Pará, Brazil), S. taeniurus from Novo Airão (Negro River, Amazonas, Brazil), S. kneri (Caura River, Venezuela), and Prochilodus nigricans also from Piagaçu-Purus. In S. insignis observed heterozygosities ranged from 0.516 to 0.968, expected heterozygosities from 0.574 to 0.973, and the number of alleles per locus varied from 4 to 32 in 31 analyzed individuals. All loci were unlinked and three loci showed significant deviations from Hardy–Weinberg equilibrium. Most markers could be successfully transferred to the other four prochilodontids, making them potentially useful for conservation genetic studies of Semaprochilodus spp.

Keywords

Semaprochilodus insignis Flagtail prochilodus Population analysis Microsatellite loci Jaraqui 

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Kelmer Batalha Passos
    • 1
  • Adam Souza de Alencar Leão
    • 1
  • Deyla Paula de Oliveira
    • 1
  • Izeni Pires Farias
    • 1
  • Tomas Hrbek
    • 1
    • 2
  1. 1.Laboratório de Evolução e Genética Animal, Departamento de BiologiaUniversidade Federal do AmazonasManausBrazil
  2. 2.Biology DepartmentUniversity of Puerto RicoSan JuanPuerto Rico

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