Conservation Genetics Resources

, Volume 2, Supplement 1, pp 325–327 | Cite as

Anonymous nuclear markers for SouthEast Asian halfbeak fishes (Dermogenys)

  • Mark de BruynEmail author
  • Wendy Grail
  • Axel Barlow
  • Gary R. Carvalho
Technical Note


We report the development of nine single-copy anonymous nuclear polymorphic loci (SCNPs) developed from the SouthEast Asian freshwater livebearing halfbeak fish Dermogenys pusilla. Ninety-six clones from a genomic library were examined, and 43 potential loci were tested across several species of Dermogenys, and in the related halfbeak Hemirhamphodon pogonognathus. All loci reported here amplified successfully in six putative species of Dermogenys, but not in Hemirhamphodon. An average of 50 SNPs per kilobase were identified (total of 5,850 bp sequenced per individual). These markers should provide a useful resource for species delineation, and other phylogenetic, phylogeographic and population genetic studies of Dermogenys halfbeaks.


Anonymous nuclear loci Halfbeak Hemirhamphidae Teleost 



We thank Bryan Carstens and Bryce Noonan for helpful advice and protocols. This research was funded by a European Community Marie Curie Incoming International Fellowship (FP6 MIIF-CT-2006-39798) to MdB.


  1. Aljanabi SM, Martinez I (1997) Universal and rapid salt-extraction of high quality genomic DNA for PCR-based techniques. Nucleic Acids Res 25:4692–4693CrossRefPubMedGoogle Scholar
  2. Ballard JWO, Whitlock MC (2004) The incomplete natural history of mitochondria. Mol Ecol 13:729–744CrossRefPubMedGoogle Scholar
  3. Dudgeon D, Arthington AH, Gessner MO et al (2006) Freshwater biodiversity: importance, threats, status and conservation challenges. Biol Rev 81:163–182CrossRefPubMedGoogle Scholar
  4. Meisner AD (2001) Phylogenetic systematics of the viviparous halfbeak genera Dermogenys and Nomorhamphus (Teleostei: Hemirhaphidae: Zenarchopterinae). Zool J Linn Soc 133:199–283CrossRefGoogle Scholar
  5. Myers N, Mittermeier RA, Mittermeier CG et al (2000) Biodiversity hotspots for conservation priorities. Nature 403:853–858CrossRefPubMedGoogle Scholar
  6. Noonan BP, Yoder AD (2009) Anonymous nuclear markers for Malagasy plated lizards (Zonosaurus). Mol Ecol Resour 9:402–404CrossRefGoogle Scholar
  7. Rozen S, Skaletsky HJ (2000) Primer 3 on the WWW for general users and biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics, methods and protocols: methods in molecular biology. Humana Press, Totowa, pp 365–386Google Scholar
  8. Wallace AR (1859) Letter from Mr Wallace concerning the geographical distribution of birds. Ibis 1:449–454Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Mark de Bruyn
    • 1
    Email author
  • Wendy Grail
    • 1
  • Axel Barlow
    • 1
  • Gary R. Carvalho
    • 1
  1. 1.School of Biological SciencesBangor UniversityBangorUK

Personalised recommendations