Conservation Genetics Resources

, Volume 2, Issue 1, pp 139–141 | Cite as

Isolation and characterization of 17 polymorphic microsatellite loci in the kangaroo mouse, genus Microdipodops (Rodentia: Heteromyidae)

  • Stacey L. Lance
  • Jessica E. Light
  • Kenneth L. Jones
  • Cris Hagen
  • John C. Hafner
Technical Note


We isolated and characterized 17 microsatellite loci from kangaroo mice, Microdipodops megacephalus and M. pallidus. Loci were screened in 24 individuals from 21 general localities across their distributional range in the Great Basin Desert. In total, the number of alleles per locus ranged from 4 to 16, observed heterozygosity ranged from 0.333 to 1, and the probability of identity values ranged from 0.013 to 1. These loci provide new tools for examining the biogeographic history and population dynamics of Microdipodops in the context of molecular ecology.


Microdipodops Kangaroo mouse Microsatellite PCR primers SSR STR 



This work was supported in part by the Nevada Department of Wildlife (contract 08-15 to J.C.H). Animals collected for this study were treated in a humane manner following the procedures approved by the American Society of Mammalogists (Gannon et al., 2007) and Occidental College’s Institutional Animal Care and Use Committee. Manuscript preparation was partially supported by the Department of Energy under Award Number DE-FC09-07SR22506 to the University of Georgia Research Foundation.


This report was prepared as an account of work sponsored by an agency of the United States Government. Neither the United States Government nor any agency thereof, nor any of their employees, makes any warranty, express or implied, or assumes any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, apparatus, product, or process disclosed, or represents that its use would not infringe privately owned rights. Reference herein to any specific commercial product, process, or service by trade name, trademark, manufacturer, or otherwise does not necessarily constitute or imply its endorsement, recommendation, or favoring by the United States Government or any agency thereof. The views and opinions of authors expressed herein do not necessarily state or reflect those of the United States Government or any agency thereof.


  1. DeWoody AJ, Schupp J, Kenefic L et al (2004) Universal method for producing ROX-labeled size standards suitable for automated genotyping. BioTechniques 37:348–350PubMedGoogle Scholar
  2. Don RH, Cox PT, Wainwright BJ, Baker K, Mattick JS (1991) ‘Touchdown’ PCR to circumvent spurious priming during gene amplification. Nucleic Acids Res 19:4008CrossRefPubMedGoogle Scholar
  3. Faircloth BC (2008) MSATCOMMANDER: detection of microsatellite repeat arrays and automated, locus-specific primer design. Mol Ecol 8:92–94CrossRefGoogle Scholar
  4. Gannon WL, Sikes RS, The Animal Care, Use Committee of the American Society of Mammalogists (2007) Guidelines of the American society of mammalogists for the use of wild animals in research. J Mammal 88:809–823CrossRefGoogle Scholar
  5. Glenn TC, Schable NA (2005) Isolating microsatellite DNA loci. Methods Enzym 395:202–222CrossRefGoogle Scholar
  6. Hafner JC, Reddington E, Craig MT (2006) Kangaroo mice (Microdipodops megacephalus) of the Mono Basin: phylogeography of a peripheral isolate. J Mammal 87:1204–1217CrossRefGoogle Scholar
  7. Hafner JC, Light JE, Hafner DJ, Hafner MS, Reddington E, Rogers DS, Riddle BR (2007) Basal clades and molecular systematics of heteromyid rodents. J Mammal 88:1129–1145CrossRefGoogle Scholar
  8. Hafner JC, Upham NS, Reddington E, Torres CW (2008) Phylogeography of the pallid kangaroo mouse, Microdipodops pallidus: a sand-obligate endemic of the Great Basin, western North America. J Biogeogr 35:2102–2118CrossRefPubMedGoogle Scholar
  9. Peakall R, Smouse PE (2006) GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol Ecol Notes 6:288–295CrossRefGoogle Scholar
  10. Rousset F (2008) GENEPOP’007: a complete re-implementation of the GENEPOP software for Windows and Linux. Mol Ecol Res 8:103–106CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Stacey L. Lance
    • 1
  • Jessica E. Light
    • 2
  • Kenneth L. Jones
    • 3
  • Cris Hagen
    • 1
  • John C. Hafner
    • 4
  1. 1.Savannah River Ecology LaboratoryAikenUSA
  2. 2.Department of Wildlife and Fisheries SciencesTexas A&M UniversityCollege StationUSA
  3. 3.Georgia Genomics FacilityUniversity of GeorgiaAthensUSA
  4. 4.Moore Laboratory of Zoology and Department of BiologyOccidental CollegeLos AngelesUSA

Personalised recommendations