Conservation Genetics Resources

, Volume 2, Issue 1, pp 139–141 | Cite as

Isolation and characterization of 17 polymorphic microsatellite loci in the kangaroo mouse, genus Microdipodops (Rodentia: Heteromyidae)

  • Stacey L. Lance
  • Jessica E. Light
  • Kenneth L. Jones
  • Cris Hagen
  • John C. Hafner
Technical Note

Abstract

We isolated and characterized 17 microsatellite loci from kangaroo mice, Microdipodops megacephalus and M. pallidus. Loci were screened in 24 individuals from 21 general localities across their distributional range in the Great Basin Desert. In total, the number of alleles per locus ranged from 4 to 16, observed heterozygosity ranged from 0.333 to 1, and the probability of identity values ranged from 0.013 to 1. These loci provide new tools for examining the biogeographic history and population dynamics of Microdipodops in the context of molecular ecology.

Keywords

Microdipodops Kangaroo mouse Microsatellite PCR primers SSR STR 

Notes

Acknowledgments

This work was supported in part by the Nevada Department of Wildlife (contract 08-15 to J.C.H). Animals collected for this study were treated in a humane manner following the procedures approved by the American Society of Mammalogists (Gannon et al., 2007) and Occidental College’s Institutional Animal Care and Use Committee. Manuscript preparation was partially supported by the Department of Energy under Award Number DE-FC09-07SR22506 to the University of Georgia Research Foundation.

Disclaimer

This report was prepared as an account of work sponsored by an agency of the United States Government. Neither the United States Government nor any agency thereof, nor any of their employees, makes any warranty, express or implied, or assumes any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, apparatus, product, or process disclosed, or represents that its use would not infringe privately owned rights. Reference herein to any specific commercial product, process, or service by trade name, trademark, manufacturer, or otherwise does not necessarily constitute or imply its endorsement, recommendation, or favoring by the United States Government or any agency thereof. The views and opinions of authors expressed herein do not necessarily state or reflect those of the United States Government or any agency thereof.

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Stacey L. Lance
    • 1
  • Jessica E. Light
    • 2
  • Kenneth L. Jones
    • 3
  • Cris Hagen
    • 1
  • John C. Hafner
    • 4
  1. 1.Savannah River Ecology LaboratoryAikenUSA
  2. 2.Department of Wildlife and Fisheries SciencesTexas A&M UniversityCollege StationUSA
  3. 3.Georgia Genomics FacilityUniversity of GeorgiaAthensUSA
  4. 4.Moore Laboratory of Zoology and Department of BiologyOccidental CollegeLos AngelesUSA

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