Conservation Genetics Resources

, Volume 2, Supplement 1, pp 273–277 | Cite as

PCR primers for microsatellite loci in a Madagascan waterbird, the Sakalava Rail (Amaurornis olivieri)

  • Edward G. Brede
  • Peter Long
  • Sama Zefania
  • Marc Rabenandrasana
  • Tamás Székely
  • Michael Bruford
Technical Note

Abstract

We isolated 76 microsatellite loci from the Sakalava rail (Amaurornis olivieri) genome. These were tested in 30 individuals from six sites (in three regions) across the species’ range in Madagascar. Thirty-four of the 76 loci produced unambiguous polymorphic markers, with mean He = 0.544 (range 0.0678–0.885), mean Ho = 0.430 (range 0.0400–0.767) and mean Na = 4.59 (range 2–10). In addition, we tested all primers for amplification in the Wood rail, Canirallus kioloides (N = 3) and the Bemaraha rail, Canirallus sp. nov (N = 3). Twelve of the loci produced successful amplification with eight loci showing polymorphism.

Keywords

Amaurornis olivieri Canirallus kioloides Rail Waterbird Madagascar Microsatellite Primer Endangered 

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Edward G. Brede
    • 1
  • Peter Long
    • 2
  • Sama Zefania
    • 3
    • 4
  • Marc Rabenandrasana
    • 5
  • Tamás Székely
    • 2
  • Michael Bruford
    • 1
  1. 1.Biodiversity and Ecological Processes Research GroupCardiff UniversityCardiffUK
  2. 2.Biodiversity Lab, Department of Biology and BiochemistryUniversity of BathBathUK
  3. 3.Madagascar National ParksToliaraMadagascar
  4. 4.Department of BiologyUniversity of ToliaraManinday, ToliaraMadagascar
  5. 5.Development and Biodiversity Conservation Action for Madagascar (DBCAM)Antananarivo 101Madagascar

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