Characterization of ten microsatellite loci for western brook lamprey Lampetra richardsoni
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We describe the isolation and development of ten polymorphic microsatellite loci for western brook lamprey (Lampetra richardsoni). Two to nine alleles were observed per locus in a sample of 35 fish. Gene diversity ranged from 0.000 to 0.792 in six populations. Cross-species amplification was successful as nine, seven and eight loci were polymorphic in Lampetra pacifica, Entosphenus hubbsi, and Entosphenus tridentatus, respectively. These markers will aid in evaluating the population structure of lamprey that are native to western North America.
KeywordsWestern brook lamprey Microsatellites Lampetra sp. Entosphenus sp.
We thank Stewart Reid, Damon Goodman and Greg Silver for providing samples; Craig McFarlane for technical assistance; and Howard Schaller and the U.S. Fish and Wildlife Service for financial support. Mention of trade names does not imply endorsement by the USFWS. The findings and conclusions in this manuscript are those of the authors and do not represent the views of the USFWS.
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