Isolation and characterization of compound microsatellite loci in the tri-spine horseshoe crab (Tachypleus tridentatus)
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The tri-spine horseshoe crab, Tachypleus tridentatus, known as “a living fossil”, is distributed from coastal Southeast Asia to western Japan. The Japanese population represents the northernmost range for this species. As is probably the case for almost of all the Asian T. tridentatus populations, in Japan it is facing near extinction and lies in urgent need of protective conservation measures. To examine the genetic structure and the genetic diversity of the Japanese population, eight novel compound microsatellite loci were developed by the intercompound microsatellite method. The number of alleles ranged from 2 to 4 per locus. The observed and expected heterozygosities were between 0.38 and 0.66, and between 0.36 and 0.67, respectively. All 32 individuals analyzed had unique genotype with 8 loci and were considered useful as conservation genetic markers for this endangered species.
KeywordsCompound microsatellite Horseshoe crab Japan Tachypleus tridentatus
We thank the following institutions and individuals for their supports and collection of samples: Fukuoka branch of the Japan Kabutogani wo Mamoru Kai (Society of conservation for horseshoe crab, Japan), the Marine World Umino-Nakamichi, Kyushu Environmental Evaluation Association, Environmental Bureau of Fukuoka City, Michiyama, A., Kido, Y., Shibata, K., Mansyo, M., Hamada, M and C. Wood. This work was supported in part by Global COE Program (Center of excellence for Asian conservation ecology as a basis of human-nature mutualism), MEXT, Japan.
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