Conservation Genetics Resources

, Volume 2, Supplement 1, pp 31–33 | Cite as

Isolation and characterization of compound microsatellite loci in the tri-spine horseshoe crab (Tachypleus tridentatus)

Technical Note


The tri-spine horseshoe crab, Tachypleus tridentatus, known as “a living fossil”, is distributed from coastal Southeast Asia to western Japan. The Japanese population represents the northernmost range for this species. As is probably the case for almost of all the Asian T. tridentatus populations, in Japan it is facing near extinction and lies in urgent need of protective conservation measures. To examine the genetic structure and the genetic diversity of the Japanese population, eight novel compound microsatellite loci were developed by the intercompound microsatellite method. The number of alleles ranged from 2 to 4 per locus. The observed and expected heterozygosities were between 0.38 and 0.66, and between 0.36 and 0.67, respectively. All 32 individuals analyzed had unique genotype with 8 loci and were considered useful as conservation genetic markers for this endangered species.


Compound microsatellite Horseshoe crab Japan Tachypleus tridentatus 


  1. Itow T (1993) Crisis in the Seto Inland Sea: the decimation of the horseshoe crab. EMECS Newslett 3:10–11Google Scholar
  2. Lian CL, Wadud MA, Geng QF, Shimatani K, Hogetsu T (2006) An improved technique for isolating codominant compound microsatellite markers. J Plant Res 119:415–417CrossRefPubMedGoogle Scholar
  3. Ministry of the Environment (2006) Threatened wildlife of Japan Red Data Book, vol 7, 2nd edn. Arthropods, Japan Wildlife Research Center, TokyoGoogle Scholar
  4. Nishida S, Koike H (2009) Genetic structure of Japanese populations of Tachypleus tridentatus by mtDNA AT-rich region sequence analysis. In: Tanacredi J, Botton M, Smith D (eds) Biology and conservation of horseshoe crabs. Springer, New York, pp 183–196CrossRefGoogle Scholar
  5. Rousset F (2008) Genepop’007: a complete reimplementation of the Genepop software for Windows and Linux. Mol Ecol Resour 8:103–106CrossRefGoogle Scholar
  6. Sekiguchi K (1988) Biology of horseshoe crabs. Science House, TokyoGoogle Scholar
  7. van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) Micro-Checker: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538CrossRefGoogle Scholar
  8. Wu B, Shiokawa A, Matsushita N, Hogetsu T (2008) Isolation of compound microsatellite loci in the herbaceous perennial Cirsium purpuratum (Maxim.) Matsum. Mol Ecol Resour 8:814–817CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  1. 1.Department of Biodiversity, Graduate School of Social and Cultural StudiesKyushu UniversityFukuokaJapan

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