Isolation and characterization of compound microsatellite loci in the tri-spine horseshoe crab (Tachypleus tridentatus)
The tri-spine horseshoe crab, Tachypleus tridentatus, known as “a living fossil”, is distributed from coastal Southeast Asia to western Japan. The Japanese population represents the northernmost range for this species. As is probably the case for almost of all the Asian T. tridentatus populations, in Japan it is facing near extinction and lies in urgent need of protective conservation measures. To examine the genetic structure and the genetic diversity of the Japanese population, eight novel compound microsatellite loci were developed by the intercompound microsatellite method. The number of alleles ranged from 2 to 4 per locus. The observed and expected heterozygosities were between 0.38 and 0.66, and between 0.36 and 0.67, respectively. All 32 individuals analyzed had unique genotype with 8 loci and were considered useful as conservation genetic markers for this endangered species.
KeywordsCompound microsatellite Horseshoe crab Japan Tachypleus tridentatus
- Itow T (1993) Crisis in the Seto Inland Sea: the decimation of the horseshoe crab. EMECS Newslett 3:10–11Google Scholar
- Ministry of the Environment (2006) Threatened wildlife of Japan Red Data Book, vol 7, 2nd edn. Arthropods, Japan Wildlife Research Center, TokyoGoogle Scholar
- Sekiguchi K (1988) Biology of horseshoe crabs. Science House, TokyoGoogle Scholar