Advertisement

Conservation Genetics Resources

, Volume 2, Supplement 1, pp 229–231 | Cite as

Isolation and characterization of 12 polymorphic tetranucleotide microsatellite loci in the apostlebird (Struthidea cinerea)

  • L. A. Rollins
  • C. E. Holleley
  • J. Wright
  • A. F. Russell
  • S. C. Griffith
Technical Note

Abstract

The apostlebird (Struthidea cinerea) is an Australian endemic passerine belonging to the Corcoracidae family. The species is highly gregarious throughout the year and the name of the species refers to the apparent prevalence of social groups of around 12 birds. The species is becoming a model system for the study of sociality in vertebrates, which will require the analysis of relatedness, paternity and maternity. We characterize 12 microsatellite loci tested for polymorphism on 25 individuals from a population in western New South Wales, Australia. The number of alleles ranged from 4 to 9 per locus. Expected heterozygosities ranged from 0.69 to 0.88. This microsatellite panel will facilitate future studies that will advance our understanding of dispersal processes, inbreeding avoidance and reproductive skew in social animals.

Keywords

Apostlebird Microsatellite Struthidea cinerea Corcoracidae Primers 

Notes

Acknowledgments

We thank Barbara Hefti-Gautschi from Ecogenics GmbH (Zurich, Switzerland). This work was funded by grants from the Australian Research Council to AFR and SCG.

References

  1. Armour JAL, Neumann R, Gobert S, Jeffreys AJ (1994) Isolation of human simple repeat loci by hybridization selection. Hum Mol Genet 3:599–605CrossRefPubMedGoogle Scholar
  2. Chapman G (1998) The social life of the apostelbird Struthidea cinerea. Emu 98:178–183CrossRefGoogle Scholar
  3. Excoffier L, Laval G, Schneider S (2005) Arlequin ver. 3.0: an integrated software package for population genetics data analysis. Evol Bioinform Online 1:47–50PubMedGoogle Scholar
  4. Gautschi B, Tenzer I, Müller JP, Schmid B (2000a) Isolation and characterization of microsatellite loci in the bearded vulture (Gypaetus barbatus) and cross-amplification in three old world vulture species. Mol Ecol 9:2193–2195CrossRefPubMedGoogle Scholar
  5. Gautschi B, Widmer A, Koella J (2000b) Isolation and characterization of microsatellite loci in the dice snake (Natrix tessellata). Mol Ecol 9:2192–2193CrossRefGoogle Scholar
  6. Griesser M, Barnaby J, Schneider NA, Figenschau N, Wright J, Griffith SC, Kazem A, Russell AF (2009) Influence of winter ranging behaviour on the social organization of a cooperatively breeding bird species, the apostlebird. Ethology 115:888–896CrossRefGoogle Scholar
  7. Heinsohn R, Cockburn A (1994) Helping is costly to young birds in cooperatively breeding white-winged choughs. Proc R Soc Lond B 256:293–298CrossRefGoogle Scholar
  8. Neilan BA, Wilton AN, Jacobs D (1997) A universal procedure for primer labelling of amplicons. Nucleic Acids Res 25:2938–2939CrossRefPubMedGoogle Scholar
  9. Schuelke M (2000) An economic method for the fluorescent labeling of PCR fragments. Nat Biotechnol 18:233–234CrossRefPubMedGoogle Scholar
  10. Woxwold IA (2004) Breeding ecology and group dynamics of the apostlebird. Aust J Zool 52:561–581CrossRefGoogle Scholar
  11. Woxwold IA, Mulder RA, Magrath MJL (2006a) Contributions to care vary with age, sex, breeding status and group size in the cooperatively breeding apostlebird. Anim Behav 72:63–73CrossRefGoogle Scholar
  12. Woxwold IA, Adcock GJ, Mulder RA (2006b) Fine-scale genetic structure and dispersal in cooperatively breeding apostlebirds. Mol Ecol 15:3139–3146CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • L. A. Rollins
    • 1
  • C. E. Holleley
    • 1
  • J. Wright
    • 2
  • A. F. Russell
    • 1
    • 3
  • S. C. Griffith
    • 1
  1. 1.Department of Brain, Behaviour and EvolutionMacquarie UniversitySydneyAustralia
  2. 2.Institute of BiologyNorwegian University of Science and TechnologyTrondheimNorway
  3. 3.Department of Animal and Plant SciencesUniversity of SheffieldSheffieldUK

Personalised recommendations