Isolation and characterization of microsatellite loci for the study of Mexican howler monkeys, their natural hybrids, and other Neotropical primates
Here we present 26 microsatellite markers that can be used for the study of Alouatta palliata, A. pigra and their natural hybrids. Sixteen of these markers were isolated for other species of primates and some of these have been previously tested in A. palliata and/or A. pigra individuals. The other 10 loci were isolated from either A. palliata or A. pigra through enriched genomic libraries. Sixteen loci were polymorphic for A. palliata and 24 for A. pigra. To explore the potential use of these primers in other primate species, we attempted cross-amplification in six other Neotropical primate taxa. Twenty-five loci amplified for at least one other species of Neotropical primate.
KeywordsSSRs Hybridization Alouatta Ateles Cebus
This work was supported by the National Science Foundation (grant DEB-0640519, PEPC and Physical Anthropology programs), and the University of Michigan (OVPR grant U014374), both to LCO. Laboratory work was conducted in the Genomic Diversity Laboratory of the Museum of Zoology, University of Michigan and in the laboratory of Dr. Eldredge Berminghan at the Smithsonian Tropical Research Institute. LCO and a team from the Universidad Veracruzana, headed by Domingo Canales Espinosa collected samples from Mexican primates, under proper collecting permits by the Mexican government. Panamanian Ateles and Cebus samples were collected at Summit Zoo. Other Alouatta samples were kindly provided by Manuel Ruiz García and María Clara Domínguez. LCO acknowledges SEMARNAT (Mexico), ANAM (Panama) and USFWS (US) for collecting, exportation, and importation permits. Thomas Duda, Jr. provided useful comments to improve the manuscript.
- CITES (2009) Convention on international trade in endangered species of wild fauna and flora. The CITES Appendices http://www.cites.org/eng/app/appendices.shtml . Accessed 11 Aug 2009
- IUCN (2009) IUCN red list of threatened species. Version 2009.1. www.iucnredlist.org. Accessed 11 Aug 2009
- Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecuminicisms. J Hered 86:248–249Google Scholar