DNA microsatellite markers for “dourada” (Brachyplatystoma rousseauxii, Siluriformes: Pimelodidae), a migratory catfish of utmost importance for fisheries in the Amazon: development, characterization and inter-specific amplification
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The long-distance migratory catfish Brachyplatystoma rousseauxii is among the two most important commercial catfish in the Amazon region. Recent data lists this species as being affected by overfishing. Thirty polymorphic microsatellite loci were isolated and characterized in 35 individuals. The number of alleles per locus ranged from 2 to 22, with an average of 9.87. The observed (H O) and the expected heterozygosity (H E) ranged between 0.143–0.914 (average 0.636) and 0.215–0.929 (average 0.706), respectively. Five loci showed a significant deviation from the Hardy–Weinberg Equilibrium and linkage disequilibrium was detected between loci BR68/BR44, BR68/BR48 and BR68/BR61. Cross-amplification in six con-generic species resulted between 12 and 21 polymorphic loci, depending upon the species. These polymorphic microsatellites should be useful for management policies of B. rousseauxii in the Amazon as well as for the others Brachyplatystoma species of commercial value.
KeywordsBrachyplatystoma rousseauxii Migratory catfish Microsatellite markers Amazon Dourada Siluriformes
This work was supported by grants FAPEAM/PIPT (205/03) and CNPq/CT-Amazônia (554054/2006-0) to JSB, FAPEAM/Temático (903/2003) to JAA-G., CNPq/PPG-7 (557090/2005) to IPF, and PROCAD-Amazônia-INPA/UNICAMP/UFRGS/CAPES (023/2006). Collections were performed under permissions numbers 130 and 14278-1 from IBAMA. The authors thank Anete Souza (CBMEG/UNICAMP) for crucial lab assistance and Giselle Guimarães for help in several phases of this study. We also thank Vera Val (LEEM/INPA) for making the sequencing on the ABI 3130 available. JSB was supported by PosGrad/FAPEAM doctoral fellowship.
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