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Polymorphic microsatellite loci for Stylosanthes macrocephala Ferr. et Costa, a tropical forage legume

  • M. O. Santos
  • R. P. Sassaki
  • T. H. S. Ferreira
  • R. M. S. Resende
  • L. Chiari
  • C. T. Karia
  • F. G. Faleiro
  • L. Jungmann
  • M. I. Zucchi
  • A. P. SouzaEmail author
Technical Note

Abstract

Microsatellites were developed for Stylosanthes macrocephala, aiming at developing tools for studying the genetic diversity of this species. A total of 13 polymorphic microsatellite markers were isolated from a S. macrocephala enriched genomic library. The isolated microsatellites were characterized in 20 accessions of the S. macrocephala germplasm collection belonging to the Empresa Brasileira de Pesquisa Agropecuária (Embrapa) Cerrados. The number of alleles per locus varied from 2 to 11, with an average of 4 alleles per locus. The observed and expected heterozygosities ranged from 0 to 0.25 and 0.05 to 0.90, respectively. Cross-amplification of the S. macrocephala polymorphic microsatellites was evaluated in three other Stylosanthes species. The microsatellites reported herein are the first set of microsatellite markers developed for S. macrocephala and are potentially useful for further studies on genetic diversity, conservation and breeding of this species.

Keywords

Molecular markers Genetic diversity Genomic enriched library 

Notes

Acknowledgments

The authors are grateful to Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) for financial support to this research (Grant # 2005/51010-0). M.O.S. received a scholarship from FAPESP (2005/52211-9) and A.P.S. is recipient of a research fellowship from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • M. O. Santos
    • 1
  • R. P. Sassaki
    • 1
  • T. H. S. Ferreira
    • 1
  • R. M. S. Resende
    • 2
  • L. Chiari
    • 2
  • C. T. Karia
    • 3
  • F. G. Faleiro
    • 3
  • L. Jungmann
    • 2
  • M. I. Zucchi
    • 4
  • A. P. Souza
    • 1
    Email author
  1. 1.Centro de Biologia Molecular e Engenharia GenéticaUniversidade Estadual de CampinasCampinasBrazil
  2. 2.Empresa Brasileira de Pesquisa Agropecuária (Embrapa) – Gado de CorteCampo GrandeBrazil
  3. 3.Empresa Brasileira de Pesquisa Agropecuária (Embrapa) – CerradosPlanaltinaBrazil
  4. 4.Pólo Apta Centro Sul – RodPiracicabaBrazil

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