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Development of microsatellite markers for Brachiaria humidicola (Rendle) Schweick

  • L. Jungmann
  • B. B. Z. Vigna
  • J. Paiva
  • A. C. B. Sousa
  • C. B. do Valle
  • P. R. Laborda
  • M. I. Zucchi
  • A. P. de Souza
Technical Note

Abstract

We describe the first panel of nuclear simple sequence repeats (SSRs) loci for Brachiaria humidicola (Rendle) Schweick., a warmseason grass with facultative apomixis, variation in ploidy levels (6X–9X), and important forage grass species in the Tropics. Of 38 pairs of primers obtained by using an enriched-library methodology, 27 revealed polymorphism in 58 accessions of the Germplasm Collection of B. humidicola held at Embrapa Beef Cattle, Brazil. Eleven loci amplified in B. dictyoneura, a closely related species with unclear taxonomic boundaries with B. humidicola. Transferability to other three Brachiaria species was also evaluated. The developed microsatellites are potentially useful for genetic studies of B. humidicola, as well as phylogenetic evaluations, conservation and breeding applications.

Keywords

Urochloa humidicola Koroviniagrass SSRs Taxonomy Genetic diversity 

Notes

Acknowledgements

The authors would like to thank Felipe Rodrigues da Silva for assisting with bioinformatics. This work was supported by grants from Brazilian Agricultural Research Corporation (EMBRAPA), Brazilian National Council for Scientific and Technological Development (CNPq), State of São Paulo Research Foundation (FAPESP) and Foundation for Science and Technological Development of the State of Mato Grosso do Sul (FUNDECT). The authors are also grateful to FUNDECT for graduate fellowship to Jungmann, L., FAPESP for undergraduate fellowships to Paiva, J. and Vigna, B.B.Z. and graduate fellowships to Sousa, A. C. B. and Laborda, P, R. and, to CNPq for a research fellowship awarded to Souza, A. P.

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • L. Jungmann
    • 1
  • B. B. Z. Vigna
    • 2
  • J. Paiva
    • 2
  • A. C. B. Sousa
    • 2
  • C. B. do Valle
    • 1
  • P. R. Laborda
    • 2
  • M. I. Zucchi
    • 3
  • A. P. de Souza
    • 2
    • 4
  1. 1.Plant Biotechnology LaboratoryEMBRAPA Beef CattleCampo GrandeBrazil
  2. 2.Center for Molecular Biology and Genetic EngineeringUniversity of Campinas (UNICAMP)CampinasBrazil
  3. 3.Agronomic Institute of Campinas (IAC)CampinasBrazil
  4. 4.Biology Institute, Plant Biology DepartmentUniversity of Campinas (UNICAMP)CampinasBrazil

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