Characterization and PCR multiplexing of 14 new polymorphic microsatellite loci for the invasive subantarctic carabid Merizodus soledadinus (Coleoptera: Carabidae)
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Abstract
Seventeen novel microsatellite markers were characterized from an enriched DNA library for the invasive carabid beetle Merizodus soledadinus. Loci were tested, as parts of two multiplex sets in 48 M. soledadinus from 2 populations. 14 loci gave reliable amplification, and genetic diversity ranged from 2 to 6 alleles per locus. In other species of the supertribe trechitae, 8 loci reliably amplified in Eotachys bistriatus, whereas all failed for Trechisibus antarcticus and Trechus obtusus.
Keywords
Microsatellite Multiplex PCR Carabid Sub-Antarctic Merizodus soledadinusNotes
Acknowledgments
This research was funded by the Institut Polaire Francais (program Institut Paul Emile Victor 136; coordinator: Marc Lebouvier) and the Agence Nationale de la Recherche (ANR-07-VULN-004, «EVINCE»). We thank Dr. Peter Convey, Dr. Roger Worland (British Antarctic Survey, Cambridge, UK), and Dr. José Retamales, Dr. Marcelo González and Carla Gimpel (Chilean Antarctic Institut, INACH, Chile), as well as Jacques Coulon (Société Linnéenne de Lyon) for providing Trechus obtusus and Eotachys bistriatus.
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