Development and characterization of 38 novel EST-SSRs for the sea cucumber Apostichopus japonicus

  • Wei Peng
  • Zhenmin Bao
  • Huixia Du
  • Ying Dong
  • Yameng Lv
  • Zunchun Zhou
  • Jingjie Hu
Technical Note

Abstract

A total of 5728 sequences from the expressed sequence tag database of sea cucumber Apostichopus japonicus were mined for microsatellite sequences. A total of 151 ESTs containing microsatellites were identified. Of 93 primer pairs designed, 56 amplified expected products and 38 showed polymorphism in 48 individuals tested. The number of alleles ranged from 2 to 10, and the observed and expected heterozygosities varied from 0.000 to 0.900 and from 0.332 to 0.884, respectively. No significant linkage disequilibrium (LD) between pairs of loci was found and 21 loci significantly deviated from the Hardy–Weinberg equilibrium (HWE).

Keywords

EST Microsatellite Sea cucumber Apostichopus japonicus 

References

  1. Chen J (2003) Overview of sea cucumber farming and sea ranching practices in China. SPC Beche-de-Mer Inform Bull 18:18–23Google Scholar
  2. Chen L, Li Q (2007) Identification and characterization of microsatellite markers derived from expressed sequence tags of the sea cucumber Stichopus japonicus. Mol Ecol Notes 7:1057–1059CrossRefGoogle Scholar
  3. Kanno M, Li Q, Kijima A (2005) Isolation and characterization of twenty microsatellite loci in Japanese sea cucumber (Stichopus japonicus). Mar Biol 7:179–183Google Scholar
  4. Van Oosterhout C, Hutchinson WF, Wills DPM et al (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite. Mol Ecol Notes 4:535–538CrossRefGoogle Scholar
  5. Zhan A, Bao Z, Hu J et al (2005) Microsatellite markers derived from bay scallop Argopecten irradians expressed sequence tags. Fisheries Sci 71:1341–1346CrossRefGoogle Scholar
  6. Zhan A, Bao Z, Hu J et al (2007) Development and characterization of 45 novel microsatellite markers for sea cucumber (Apostichopus japonicus). Mol Ecol Notes 7:1345–1348CrossRefGoogle Scholar
  7. Zhou Z, Zou L, Dong Y et al (2008) Characterization of 28 polymorphic microsatellites for Japanese sea urchin (Strongylocentrotus intermedius) via mining EST database of relative species (S. purpuratus). Ann Zool Fenn 45:181–184Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Wei Peng
    • 1
  • Zhenmin Bao
    • 1
  • Huixia Du
    • 1
  • Ying Dong
    • 2
  • Yameng Lv
    • 1
  • Zunchun Zhou
    • 2
  • Jingjie Hu
    • 1
  1. 1.Laboratory of Marine Genetics and Breeding, College of Marine Life SciencesOcean University of ChinaQingdaoChina
  2. 2.Liaoning Key Lab of Marine Fishery Molecular BiologyLiaoning Ocean and Fisheries Science Research InstituteDalianChina

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