Isolation and characterization of microsatellites loci for “piramutaba” (Brachyplatystoma vaillantii, Siluriformes: Pimelodidae), one of the commercially most important migratory catfishes in the Amazon Basin
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The piramutaba (Brachyplatystoma vaillantii) is one of the largest and most important migratory catfishes of the Amazon Basin. Due to its historical commercial importance, this species is now considered overfished. Fifteen polymorphic microsatellite markers were characterized in order to be used as tools to address aspects of the biology and conservation of this species. The number of alleles varied from 4 to 26 per locus, with an average of 11.33 alleles per locus, in 36–48 individuals tested. The observed and expected heterozygosities varied from 0.30176 to 0.95281, and from 0.25532 to 0.97778, respectively. All the microsatellites were found to be in Hardy–Weinberg equilibrium, and no linkage disequilibrium was observed. The transferability of these markers was tested in others Brachyplatystoma with seven to 12 loci being polymorphic per species. The results indicate that these markers are adequate for genetic studies applied to the management and conservation of the piramutaba, and others Brachyplatystoma species.
KeywordsPiramutaba Microsatellites Brachyplatystoma vaillantii Migratory catfish Amazon
This research was supported by grants from CNPq/PPG-7 (557090/05) to I.P.F., FAPEAM (903/03) to J.A.A.G. and CNPq/CT Amazônia (554054/06-0) to J.S.B. Permission to conduct fieldwork and collect tissue samples was granted by IBAMA (Licença no. 14278-1 and no. 130). The authors would like to especially thank Kyara Formiga for her crucial help during several phases of this project. The Projeto Pirada (LTBM/INPA) provided samples and support in the laboratory. We are grateful to Tomas Hrbek for technical assistance and manuscript review. This study forms a portion of a dissertation of F.C.R. at the Genética, Conservação e Biologia Evolutiva program of INPA. F.C.R. was supported by a fellowship from CAPES/FAPEAM.
- Almeida OT (2006) A indústria pesqueira na Amazônia. In: IBAMA/Pro Várzea CEE (ed) IBAMA/Pro Várzea, Manaus, p 10Google Scholar
- Alonso JC, Pirker LEM (2005) Dinâmica populacional e estado atual da exploração de piramutaba e de dourada. In: Fabré NN, Barthem RB (eds) O manejo da pesca dos grandes bagres migradores: piramutaba e dourada no eixo Solimões-Amazonas. IBAMA/Pro Várzea, Coleção Estudos Estratégicos, ManausGoogle Scholar
- Barthem R, Goulding M (1997) Os Bagres Balizadores: Ecologia, Migração e Conservação de Peixes Amazônicos. Sociedade Civil Mamirauá, MCT—CNPq, IPAAM, Brasilia, BrazilGoogle Scholar
- Barthem RB, Goulding M (2007) An unexpected ecosystem: the Amazon revealed by the fisheries. Gráfica Biblos, Lima/Botanical Garden Press, MOGoogle Scholar
- Batista JS, Formiga K, Farias IP, Alves-Gomes JA (2004) Genetic variability studies of piramutaba (Brachyplatistoma vaillantii) and dourada (B. rousseauxii) (Pimelodidae: Siluriformes) in the Amazon: basis for management and conservation. International Congress on the Biology of Fish, Manaus, AM, BrazilGoogle Scholar
- Nelson JS (2006) Fishes of the world, 4th edn. Wiley, New York, NYGoogle Scholar
- Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, NJGoogle Scholar
- Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Springs Harbor Laboratory Press, Cold Springs Harbor, NYGoogle Scholar