Development and characterization of microsatellite markers in Astronotus crassipinis (Heckel, 1840)
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Astronotus crassipinis is an important freshwater fish in the Amazon basin. Little is known about their population structure and genetic diversity. They live in Amazon varzea lakes and are territorial fish practicing mouth brood. Studies involving populations of A. crassipinis are of great importance for the conservation and management of this species. Therefore, thirteen microsatellite markers were developed for this species and characterized in 30 accessions from lakes Preto and Ananá in Solimões river and lake Piranha, located in the system Negro–Solimões. The observed and expected heterozygosities ranged from 0.00 to 0.72 and 0.25 to 0.58, respectively. Alleles per locus varied from two to six, with an average of three. Three loci departed significantly from Hardy–Weinberg Equilibrium (P < 0.05) after Bonferroni correction. The value of Fis (f) ranged from −0.666 to 0.909 (average on −0.133). This new set of microsatellites will contribute towards studies of genetic diversity and conservation of A. crassipinis.
KeywordsMolecular markers Single sequence repeat (SSR) Genetic diversity Conservation Astronotus crassipinis
The authors would like to thanks Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Fundação de Amparo à Pesquisa do Amazonas (FAPEAM) and Conselho Nacional de Pesquisa (CNPQ) for financial supports. CFSS was the recipient of a MSc fellowship from CAPES. CHA is the recipient of a PhD fellowship from CNPQ. The Program for Academic Cooperation PROCAD between INPA and UNICAMP was supported by CAPES. VMFAV and IPF are the recipient of research fellowship from CNPQ.
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