Development and characterization of microsatellite markers in Astronotus crassipinis (Heckel, 1840)
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Astronotus crassipinis is an important freshwater fish in the Amazon basin. Little is known about their population structure and genetic diversity. They live in Amazon varzea lakes and are territorial fish practicing mouth brood. Studies involving populations of A. crassipinis are of great importance for the conservation and management of this species. Therefore, thirteen microsatellite markers were developed for this species and characterized in 30 accessions from lakes Preto and Ananá in Solimões river and lake Piranha, located in the system Negro–Solimões. The observed and expected heterozygosities ranged from 0.00 to 0.72 and 0.25 to 0.58, respectively. Alleles per locus varied from two to six, with an average of three. Three loci departed significantly from Hardy–Weinberg Equilibrium (P < 0.05) after Bonferroni correction. The value of Fis (f) ranged from −0.666 to 0.909 (average on −0.133). This new set of microsatellites will contribute towards studies of genetic diversity and conservation of A. crassipinis.
KeywordsMolecular markers Single sequence repeat (SSR) Genetic diversity Conservation Astronotus crassipinis
The authors would like to thanks Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Fundação de Amparo à Pesquisa do Amazonas (FAPEAM) and Conselho Nacional de Pesquisa (CNPQ) for financial supports. CFSS was the recipient of a MSc fellowship from CAPES. CHA is the recipient of a PhD fellowship from CNPQ. The Program for Academic Cooperation PROCAD between INPA and UNICAMP was supported by CAPES. VMFAV and IPF are the recipient of research fellowship from CNPQ.
- Allendorf FW, Luikart G (2007) Conservation and the genetics of populations. Blackwell Publishing, Oxford 641Google Scholar
- Billotte N, Lagoda PJR, Risterucci AM, Baurens FC (1999) Microsatellite-enriched libraries: applied methodology for the development of SSR markers in tropical crops. Fruits 54:277–288Google Scholar
- Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98Google Scholar
- Kullander SO (2003) Family Cichlidae (Cichlids). In: Reis RE, Kullander SO, Ferraris CJ (eds) Check list of the freshwater fishes of South and Central America. EDIPUCRS, Porto AlegreGoogle Scholar
- Miller MP (1997) Tools for population genetic analysis (TFPGA) 1.3. A windows program for the analysis of allozyme and molecular population genetic data. Computer software distributed by author. Available at http://herb.bio.nau.edul~miller/tfpga.htm
- Porto JIR, Gomes JAA, Farias IP, Feldberg E (2001) Using molecular biology techniques to characterize the diversity of Amazonian ornamental fishes. In: Chao NL, Petri P, Prang G, Soneschien L, Tlusty M (eds) Conservation and management of ornamental fish resources of the Rio Negro Basin, Amazonia, Brazil (Project Piaba). Universidade do Amazonas Press, ManausGoogle Scholar
- Reis RE, Kullander SO, Ferraris CJ (2003) Check list of the freshwater fishes of South and Central America. EDIPUCRS, Porto Alegre, BrazilGoogle Scholar
- Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, New YorkGoogle Scholar