Isolation and characterization of 14 dinucleotide microsatellite loci in the endangered herb Saruma henryi Oliv. (Aristolochiaceae)

  • Shan-Shan Dong
  • Xuan Huang
  • Zeng-Qiang Qian
  • Tian-Hua Zhou
  • Shan Li
  • Gui-Fang Zhao
Technical Note

Abstract

Saruma henryi Oliv. (Aristolochiaceae) is an endangered perennial herb endemic to China, and has received considerable attention for its high phylogenetic, ecological and medicinal significances. To investigate its genetic diversity and structure, 14 polymorphic microsatellite loci were isolated and characterized from a dinucleotide-enriched genomic library of S. henryi. The number of alleles per locus varied between 5 and 8, with the average of 7.07 per locus. The expected and observed heterozygosities ranged from 0.6281 to 0.8532 and from 0.4762 to 0.8000, respectively. In addition, their cross-taxa transferability was also evaluated in two cofamilial species Aristolochia contorta Bge. and Asarum himalaicum Hook. f. et Thoms. ex Klotzsch. These informative microsatellite loci provide a powerful tool for genetic analyses of S. henryi and related taxa.

Keywords

Aristolochiaceae Codominant markers Endemic species Microsatellites Saruma henryi Oliv. 

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Shan-Shan Dong
    • 1
  • Xuan Huang
    • 1
    • 2
  • Zeng-Qiang Qian
    • 3
  • Tian-Hua Zhou
    • 1
  • Shan Li
    • 4
  • Gui-Fang Zhao
    • 1
  1. 1.Key Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education), College of Life SciencesNorthwest UniversityXi’anPeoples Republic of China
  2. 2.Provincial Key Laboratory of Biotechnology, College of Life SciencesNorthwest UniversityXi’anPeoples Republic of China
  3. 3.School of Marine and Tropical Biology and Comparative Genomics CentreJames Cook UniversityTownsvilleAustralia
  4. 4.College of Life Sciences and TechnologyTongji UniversityShanghaiPeoples Republic of China

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