Isolation and characterization of 14 dinucleotide microsatellite loci in the endangered herb Saruma henryi Oliv. (Aristolochiaceae)

  • Shan-Shan Dong
  • Xuan Huang
  • Zeng-Qiang Qian
  • Tian-Hua Zhou
  • Shan Li
  • Gui-Fang ZhaoEmail author
Technical Note


Saruma henryi Oliv. (Aristolochiaceae) is an endangered perennial herb endemic to China, and has received considerable attention for its high phylogenetic, ecological and medicinal significances. To investigate its genetic diversity and structure, 14 polymorphic microsatellite loci were isolated and characterized from a dinucleotide-enriched genomic library of S. henryi. The number of alleles per locus varied between 5 and 8, with the average of 7.07 per locus. The expected and observed heterozygosities ranged from 0.6281 to 0.8532 and from 0.4762 to 0.8000, respectively. In addition, their cross-taxa transferability was also evaluated in two cofamilial species Aristolochia contorta Bge. and Asarum himalaicum Hook. f. et Thoms. ex Klotzsch. These informative microsatellite loci provide a powerful tool for genetic analyses of S. henryi and related taxa.


Aristolochiaceae Codominant markers Endemic species Microsatellites Saruma henryi Oliv. 



This study was financially co-supported by the National Natural Science Foundation of China (Grant No: 30800087), the Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT) and the Postgraduate Innovation and Education Program of Northwest University (NWU) (Grant No: 08YZZ37). ZQQ was supported by the Endeavour Postgraduate Award from the Department of Education, Employment and Workplace Relations, Australia.


  1. Cheng C-Y, Yang C-S, Hwang S-M (1988) Aristolochiaceae. In: Kiu H-S, Ling Y-R (eds) Flora Reipublicae Popularis Sinicae, vol 24. Science Press, Beijing, pp 159–245Google Scholar
  2. Di W-Z, Yu Z-Y (1989) State-protected rare and endangered plants in Shaanxi Province. Northwest University Press, Xi’anGoogle Scholar
  3. Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15Google Scholar
  4. Ellis JR, Burke JM (2007) EST-SSRs as a resource for population genetic analyses. Heredity 99:125–132CrossRefPubMedGoogle Scholar
  5. Freeland JR (2005) Molecular ecology. Wiley, Chichester, West Sussex, UKGoogle Scholar
  6. Gregory MP (1956) A phyletic rearrangement in the Aristolochiaceae. Am J Bot 43:110–122CrossRefGoogle Scholar
  7. Holm S (1979) A simple sequentially rejective multiple test procedure. Scand J Stat 6:65–70Google Scholar
  8. Hsu E (2005) Saruma henryi. Aristolochiaceae. Curtis’s Bot Mag 22:200–204CrossRefGoogle Scholar
  9. Igarashi S, Fukuda H (1997) The life histories of Asian butterflies. Tokai University Press, TokyoGoogle Scholar
  10. Li S, Chang Z, Chen Y (1994) Studies on conservation biology of Saruma henryi Oliv. Acta Bot Boreal-Occident Sin 14:104–108Google Scholar
  11. Liu K, Muse SV (2005) PowerMarker: an integrated analysis environment for genetic marker analysis. Bioinformatics 21:2128–2129CrossRefPubMedGoogle Scholar
  12. Thorne RF (1974) A phylogenetic classification of the Annoniflorae. Aliso 8:147–205Google Scholar
  13. Van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538CrossRefGoogle Scholar
  14. Yu Z-Y, Li S-F (1989) Preliminary study on the rare and endangered plant species in Qinling-Bashan mountains. In: Shaanxi Academy of Sciences (ed) Biological resources in Qinling mountains and their exploitation. Scientific and Technical Documents Publishing House, Beijing, pp 5–32Google Scholar
  15. Zane L, Bargelloni L, Patarnello T (2002) Strategies for microsatellite isolation: a review. Mol Ecol 11:1–16CrossRefPubMedGoogle Scholar
  16. Zhou T-H, Qian Z-Q, Li S, Guo Z-G, Huang Z-H, Liu Z-L, Zhao G-F (2009a) Genetic diversity of the endangered Chinese endemic herb Saruma henryi Oliv. (Aristolochiaceae) and its implications for conservation. Popul Ecol. doi: 10.1007/s10144-009-0139-3
  17. Zhou T-H, Zhao G-F, Li S, Qian Z-Q (2009b) Characterization of EST-derived microsatellite loci in Saruma henryi Oliv., an endangered Chinese endemic herb. Conserv Genet Resour. doi: 10.1007/s12686-009-9016-9

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Shan-Shan Dong
    • 1
  • Xuan Huang
    • 1
    • 2
  • Zeng-Qiang Qian
    • 3
  • Tian-Hua Zhou
    • 1
  • Shan Li
    • 4
  • Gui-Fang Zhao
    • 1
    Email author
  1. 1.Key Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education), College of Life SciencesNorthwest UniversityXi’anPeoples Republic of China
  2. 2.Provincial Key Laboratory of Biotechnology, College of Life SciencesNorthwest UniversityXi’anPeoples Republic of China
  3. 3.School of Marine and Tropical Biology and Comparative Genomics CentreJames Cook UniversityTownsvilleAustralia
  4. 4.College of Life Sciences and TechnologyTongji UniversityShanghaiPeoples Republic of China

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