Isolation and characterization of 14 dinucleotide microsatellite loci in the endangered herb Saruma henryi Oliv. (Aristolochiaceae)
Saruma henryi Oliv. (Aristolochiaceae) is an endangered perennial herb endemic to China, and has received considerable attention for its high phylogenetic, ecological and medicinal significances. To investigate its genetic diversity and structure, 14 polymorphic microsatellite loci were isolated and characterized from a dinucleotide-enriched genomic library of S. henryi. The number of alleles per locus varied between 5 and 8, with the average of 7.07 per locus. The expected and observed heterozygosities ranged from 0.6281 to 0.8532 and from 0.4762 to 0.8000, respectively. In addition, their cross-taxa transferability was also evaluated in two cofamilial species Aristolochia contorta Bge. and Asarum himalaicum Hook. f. et Thoms. ex Klotzsch. These informative microsatellite loci provide a powerful tool for genetic analyses of S. henryi and related taxa.
KeywordsAristolochiaceae Codominant markers Endemic species Microsatellites Saruma henryi Oliv.
- Cheng C-Y, Yang C-S, Hwang S-M (1988) Aristolochiaceae. In: Kiu H-S, Ling Y-R (eds) Flora Reipublicae Popularis Sinicae, vol 24. Science Press, Beijing, pp 159–245Google Scholar
- Di W-Z, Yu Z-Y (1989) State-protected rare and endangered plants in Shaanxi Province. Northwest University Press, Xi’anGoogle Scholar
- Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15Google Scholar
- Freeland JR (2005) Molecular ecology. Wiley, Chichester, West Sussex, UKGoogle Scholar
- Holm S (1979) A simple sequentially rejective multiple test procedure. Scand J Stat 6:65–70Google Scholar
- Igarashi S, Fukuda H (1997) The life histories of Asian butterflies. Tokai University Press, TokyoGoogle Scholar
- Li S, Chang Z, Chen Y (1994) Studies on conservation biology of Saruma henryi Oliv. Acta Bot Boreal-Occident Sin 14:104–108Google Scholar
- Thorne RF (1974) A phylogenetic classification of the Annoniflorae. Aliso 8:147–205Google Scholar
- Yu Z-Y, Li S-F (1989) Preliminary study on the rare and endangered plant species in Qinling-Bashan mountains. In: Shaanxi Academy of Sciences (ed) Biological resources in Qinling mountains and their exploitation. Scientific and Technical Documents Publishing House, Beijing, pp 5–32Google Scholar
- Zhou T-H, Qian Z-Q, Li S, Guo Z-G, Huang Z-H, Liu Z-L, Zhao G-F (2009a) Genetic diversity of the endangered Chinese endemic herb Saruma henryi Oliv. (Aristolochiaceae) and its implications for conservation. Popul Ecol. doi:10.1007/s10144-009-0139-3
- Zhou T-H, Zhao G-F, Li S, Qian Z-Q (2009b) Characterization of EST-derived microsatellite loci in Saruma henryi Oliv., an endangered Chinese endemic herb. Conserv Genet Resour. doi:10.1007/s12686-009-9016-9