Microsatellites from rubber tree (Hevea brasiliensis) for genetic diversity analysis and cross-amplification in six Hevea wild species
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Hevea brasiliensis is native to the Amazonian rain forest and an important source of natural rubber. Twenty seven polymorphic microsatellite loci were isolated and characterized from a GA–CA enriched genomic library of H. brasiliensis. The number of alleles ranged from 2 to 20. The observed and expected heterozygosity ranged from 0.13 to 0.88 and from 0.00 to 0.89, respectively. Cross-species amplification of the markers developed for H. brasiliensis was successful in the wild Hevea species H. guianensis, H. rigidifolia, H. nitida, H. pauciflora, H. benthamiana and H. camargoana. The data indicated a high degree of sequence homology in the microsatellite flanking regions of these species. The developed SSR loci are a potential powerful tool for studies of population genetics, genetic diversity and gene flow among Hevea species.
KeywordsHevea spp. Hevea brasiliensis Microsatellite Genetic diversity Transferability Cross-species amplification
The authors thank the Brazilian Agricultural Research Corporation (EMBRAPA - Amazônia Ocidental) and the Agronomic Institute of Campinas for donating the analyzed Hevea germoplasm. The present research was financed by the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, 2007/50392-1, 2007/59804-0); L.M.S. and C.M.C. received respectively a undergraduate and graduate scholarship from FAPESP. A.P.S. and P.S.G. received a research fellowship grant from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).
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