Characterization of EST-derived microsatellite loci in Saruma henryi Oliv., an endangered Chinese endemic herb
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Saruma henryi Oliv. is an endangered perennial herb endemic to China, and possesses high phylogenetic, ecological and medicinal values. In this study, 12 polymorphic microsatellite markers were developed from publicly available expressed sequence tags (ESTs) of S. henryi. Analysis of 24 individuals from a single population showed that the number of alleles varied between 2 and 5, with an average of 3.42 per locus. However, when they were assayed against individuals from multiple populations, this value ascended to between 7 and 10, with an average of 8.42 per locus. The observed and expected heterozygosities ranged from 0.2500 to 0.7500 and from 0.4028 to 0.7648, respectively. The low within-population genetic variability (number of alleles per locus) can be attributed to the clonal growth and the predominantly selfing breeding system in this species. In addition, their cross-taxa transferability was evaluated in two cofamilial species, and polymorphic products were detected. These informative EST-derived microsatellite markers can be readily used for genetic analyses of S. henryi and related taxa.
KeywordsAristolochiaceae Codominant markers Data mining Endemic species Expressed sequence tags EST-SSRs
This study was financially co-supported by the National Natural Science Foundation of China (Grant No: 30800087), the Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT) and the Postgraduate Innovation & Education Program of Northwest University (NWU) (Grant No: 08YZZ37). ZQQ was supported by the Endeavour Postgraduate Award from the Department of Education, Employment and Workplace Relations, Australia. The data analysis was partially performed in Prof. Ross H. Crozier’s laboratory, James Cook University, Australia. Voucher specimens (S. henryi: SH-TPY-070625; A. himalaicum: AH-HH-060910; A. pulchellum: AP-XR-070724) have been deposited in the specimen room of the College of Life Sciences, Northwest University.
- Benjamini Y, Liu W (1999) A distribution-free multiple test procedure that controls the false discovery rate. Department of Statistics and O.R., Tel Aviv University, Tel AvivGoogle Scholar
- Cheng C-Y, Yang C-S, Hwang S-M (1988) Aristolochiaceae. In: Kiu H-S, Ling Y-R (eds) Flora Reipublicae Popularis Sinicae, vol 24. Science Press, Beijing, pp 159–245Google Scholar
- Di W-Z, Yu Z-Y (1989) State-protected rare and endangered plants in Shaanxi Province. Northwest University Press, Xi’anGoogle Scholar
- Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15Google Scholar
- Hamrick JL, Godt MJW (1996) Conservation genetics of endemic plant species. In: Avise JC, Hamrick JL (eds) Conservation genetics: case histories from nature. Chapman and Hall, New York, pp 281–304Google Scholar
- Igarashi S, Fukuda H (1997) The life histories of Asian butterflies. Tokai University Press, TokyoGoogle Scholar
- Li S, Chang Z, Chen Y (1994) Studies on conservation biology of Saruma henryi Oliv. Acta Bot Boreal-Occident Sin 14:104–108Google Scholar
- Thorne RF (1974) A phylogenetic classification of the Annoniflorae. Aliso 8:147–205Google Scholar
- Yu Z-Y, Li S-F (1989) Preliminary study on the rare and endangered plant species in Qinling-Bashan Mountains. In: Shaanxi Academy of Sciences (ed) Biological resources in Qinling mountains and their exploitation. Scientific and Technical Documents Publishing House, Beijing, pp 5–32Google Scholar
- Zhao H, Yang P-J, Peng Q (2005) Biological study of pistil morphology and pollination of Saruma henryi. Acta Bot Boreal-Occident Sin 25:923–927Google Scholar