Characterization of EST-derived microsatellite loci in Saruma henryi Oliv., an endangered Chinese endemic herb

  • Tian-Hua Zhou
  • Gui-Fang Zhao
  • Shan LiEmail author
  • Zeng-Qiang Qian
Technical Note


Saruma henryi Oliv. is an endangered perennial herb endemic to China, and possesses high phylogenetic, ecological and medicinal values. In this study, 12 polymorphic microsatellite markers were developed from publicly available expressed sequence tags (ESTs) of S. henryi. Analysis of 24 individuals from a single population showed that the number of alleles varied between 2 and 5, with an average of 3.42 per locus. However, when they were assayed against individuals from multiple populations, this value ascended to between 7 and 10, with an average of 8.42 per locus. The observed and expected heterozygosities ranged from 0.2500 to 0.7500 and from 0.4028 to 0.7648, respectively. The low within-population genetic variability (number of alleles per locus) can be attributed to the clonal growth and the predominantly selfing breeding system in this species. In addition, their cross-taxa transferability was evaluated in two cofamilial species, and polymorphic products were detected. These informative EST-derived microsatellite markers can be readily used for genetic analyses of S. henryi and related taxa.


Aristolochiaceae Codominant markers Data mining Endemic species Expressed sequence tags EST-SSRs 



This study was financially co-supported by the National Natural Science Foundation of China (Grant No: 30800087), the Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT) and the Postgraduate Innovation & Education Program of Northwest University (NWU) (Grant No: 08YZZ37). ZQQ was supported by the Endeavour Postgraduate Award from the Department of Education, Employment and Workplace Relations, Australia. The data analysis was partially performed in Prof. Ross H. Crozier’s laboratory, James Cook University, Australia. Voucher specimens (S. henryi: SH-TPY-070625; A. himalaicum: AH-HH-060910; A. pulchellum: AP-XR-070724) have been deposited in the specimen room of the College of Life Sciences, Northwest University.


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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Tian-Hua Zhou
    • 1
  • Gui-Fang Zhao
    • 1
  • Shan Li
    • 2
    Email author
  • Zeng-Qiang Qian
    • 3
  1. 1.Key Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education), College of Life SciencesNorthwest UniversityXi’anPeople’s Republic of China
  2. 2.College of Life Sciences and TechnologyTongji UniversityShanghaiPeople’s Republic of China
  3. 3.School of Marine and Tropical Biology and Comparative Genomics CentreJames Cook UniversityTownsvilleAustralia

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