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Isolation and characterization of microsatellite loci in the tropical forage legume Stylosanthes guianensis (Aubl.) Sw.

  • M. O. Santos
  • C. T. Karia
  • R. M. S. Resende
  • L. Chiari
  • L. Jungmann
  • M. I. Zucchi
  • A. P. SouzaEmail author
Technical Note

Abstract

Stylosanthes guianensis is an important tropical pasture legume. Knowledge of genetic diversity and structure of S. guianensis populations is of great importance for the conservation and germplasm management of this species. Thus, 20 microsatellite markers were developed from a S. guianensis enriched library. The microsatellites were characterized in 20 accessions from the germplasm collection of the Empresa Brasileira de Pesquisa Agropecuária (Embrapa). The average number of alleles per locus varied from 2 to 7, with an average of 4 alleles per locus. The observed and expected heterozygosities ranged from 0 to 0.60 and 0.10 to 0.85, respectively. This new set of microsatellites will contribute towards studies of genetic diversity and conservation of S. guianensis.

Keywords

Molecular markers Genetic diversity Conservation Genomic enriched library 

Notes

Acknowledgments

The authors would like to thank Fundação de Amparo à Pesquisa do Estado de São Paulo for the financial support during the development of this research (2005/51010-0). Santos MO received a PhD scholarship from FAPESP (2005/52211-9). Souza AP is recipient of a research fellowship from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • M. O. Santos
    • 1
  • C. T. Karia
    • 2
  • R. M. S. Resende
    • 3
  • L. Chiari
    • 3
  • L. Jungmann
    • 3
  • M. I. Zucchi
    • 4
  • A. P. Souza
    • 1
    • 5
    Email author
  1. 1.Centro de Biologia Molecular e Engenharia Genética, Departamento de Genética e EvoluçãoUniversidade Estadual de CampinasCampinasBrazil
  2. 2.Empresa Brasileira de Pesquisa Agropecuária (Embrapa)—CerradosPlanaltinaBrazil
  3. 3.Empresa Brasileira de Pesquisa Agropecuária (Embrapa)—Gado de CorteCampo GrandeBrazil
  4. 4.Instituto Agronômico de Campinas (IAC)CampinasBrazil
  5. 5.Instituto de Biologia, Departamento de Biologia VegetalUniversidade Estadual de CampinasCampinasBrazil

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