Genetic identification of the sharks Rhizoprionodon porosus and R. lalandii by PCR-RFLP and nucleotide sequence analyses of 5S rDNA
- 163 Downloads
A molecular approach based on nuclear 5S rDNA sequence variability was applied successfully to correctly identify samples from the two Rhizoprionodon species collected in the wild or sold in markets. The sequence of the non-transcribed spacer (NTS) of the 5S rDNA showed high interspecific variability and no intraspecific polymorphism, making it a useful marker for sharpnose shark identification. Polymorphisms in the NTS sequences of Rhizoprionodon sharks also created unique restriction patterns for each species after PCR-RFLP analysis. The 5S rDNA polymorphism represents a fast and non expensive tool to access species identification when rapid and unequivocal identification of shark products is needed, particularly for future management and other investigations.
KeywordsGenetic markers Non-transcribed spacer Trade monitoring Sharks Species identification
The authors are grateful to Fundação de Amparo à Pesquisa do Estado de São Paulo for financial support.
- Compagno LJV (1984) FAO species catalogue, vol 4. Sharks of the world. Part 2. Carcharhiniformes. FAO fish. Synopsis 125:251–655Google Scholar
- Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98Google Scholar
- Heist EJ, Gold JR (1999) Genetic identification of sharks in the US Atlantic large coastal shark fishery. Fish Bull 97:53–61Google Scholar
- Lessa RPT, Vooren CM, Araújo MLG, Kotas JE, Almeida PC, Rincón G, Santana FM, Gadig OBF, Sampaio C (2005) Brazilian national plan for conservation and management of elasmobranch fishes stocks. SBEEL, Recife, pp 100Google Scholar