Treated Gold Screen-Printed Electrode as Disposable Platform for Label-Free Immunosensing of Salmonella Typhimurium
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Label-free detection is a fashion manner to accomplish analysis in immunosensing field, owing to the possibility to avoid labelled antibody and enzymatic reaction time, as well. To develop a label-free immunosensor, the electrochemical impedance spectroscopy in combination with the bulk gold electrode is one of the most common approaches; however, the use of laboratory setup as well as the cost of gold bulk electrode hinders their widespread applications. Herein, we report a label-free impedimetric immunosensor based on the use of gold screen-printed electrodes for Salmonella Typhimurium, chosen as model analyte. With the aim to enhance the immunosensor electrochemical performances, different protocols were accomplished to pre-treat the working electrode surface used as platform to immobilise the immunological chain in a self-assembled monolayer configuration. Salmonella Typhimurium was detected with a linear range comprised between 103 and 107 CFU/mL, demonstrating the suitability of the treated gold-printed electrode combined with portable instrumentation as implemented electrochemical analytical tool for effective and in situ analysis.
KeywordsTreatment of gold-printed electrode Electrochemical immunosensors Portable instrument Pathogen detection
F.A. likes to acknowledge the DAAD Salmonsens 57268170 project for financial support and Prof. Ilaria Cacciotti, University of Rome “Niccolò Cusano”, for SEM analysis.
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